Aberrant expression of neurotrophic factors in the ventricular progenitor cells of infant congenitally hydrocephalic rats
Aberrant expression of neurotrophic factors in the ventricular progenitor cells of infant congenitally hydrocephalic rats
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DOI：
10.1007/s003810000305
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发表时间：
2000-08-01
影响因子：
1.4
通讯作者：
Mori, K
中科院分区：
文献类型：
--
作者：
Fukumitsu, H;Ohmiya, M;Mori, K
目的：探讨神经营养因子在HTX大鼠脑积水形成中的作用。研究方法：采用免疫组织化学方法检测6日龄先天性脑积水大鼠（HTX大鼠）大脑皮质和脑室区脑源性神经营养因子（BDNF）、神经营养因子3（NT-3）和成纤维细胞生长因子1（FGF-1）的表达。在脑积水大鼠的脑室区，强BDNF样免疫反应（LI）和弱，但显着的信号NT-3和FGF-1-LI。然而，在非HTX大鼠中未检测到显著信号。HTX和非HTX大鼠的一小部分心室细胞微管相关蛋白2呈阳性。HTX组阳性细胞突起较非HTX组明显延长，提示脑积水组部分脑室细胞异位分化为成熟神经元。结论：这种分化异常可能是神经营养因子表达异常的原因。与此相反，大脑神经元层并没有表现出神经营养因子表达的显着变化。
Objects: This study was conducted to investigate the roles of neurotrophic factors in the development of hydrocephalus in HTX rats. Methods: Expressions of brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and fibroblast growth factor (FGF)-1 were examined immunohistochemically in the cerebral cortex and ventricular zone of 6-day-old rats with congenital hydrocephalus (HTX rats). In the ventricular zone of hydrocephalic rats, potent BDNF-like immunoreactivity (-LI) and weak but significant signals for NT-3- and FGF-1-LIs were observed. However, no significant signals were detected in non-HTX rats. A small subpopulation of ventricular cells was positive for microtubule-associated protein 2 in HTX and non-HTX rats. The positive cells in the HTX rats had neurites much longer than those in the non-HTX animals, suggesting that some ventricular cells of the hydrocephalics had ectopically differentiated into mature neurons. Conclusions: This abnormal differentiation may have been responsible for the aberrant expressions of neurotrophic factors. In contrast, the cerebral neuronal layers did not show such prominent alterations in neurotrophic factor expression.