Effcacy of different spinal nerve roots for neuromodulation of micturition reflex in rats
Effcacy of different spinal nerve roots for neuromodulation of micturition reflex in rats
复制标题
不同脊神经根对大鼠排尿反射的神经调节作用
作者：
Ni jianshu;Wang xiaohu;Cao nailong;Si jiemin;Gu baojun
Electrical stimulation of peripheral nerves controlling the bladder is an alternative, nondestructive medical treatment for urinary incontinence and retention.In this study, we aimed to identify the most effcient sensory and motor spinal nerve roots involved in the micturition reflex. Unilateral L5-S2 dorsal and ventral roots were electrically stimulated, and bladder reflex contractions were recorded under isovolumetric conditions. Repeated stimulation of the L6 and S1 dorsal roots not only.abolished bladder reflex contractions but also induced a poststimulation inhibitory effect, whereas repeated stimulation of the L5 and S2 dorsal roots had no effect. Only the L6 ventral root directly caused bladder contraction when ventral roots L5-S2 were stimulated in sequence. Upon retrograde tracing using pseudorabies virus (PRV), the sacral parasympathetic nucleus of the L6 segment had more PRV-positive cells than the other segments, though the S1 segment of the dorsal root ganglia had the highest density of PRV-positive neurons. These results suggest the L6 ventral root is most effcient in producing detrusor muscle contraction, and the S1 dorsal root best inhibits the micturition reflex.
