STIMULATION OF CA2+-DEPENDENT NEUROTRANSMITTER RELEASE AND PRESYNAPTIC NERVE-TERMINAL PROTEIN-PHOSPHORYLATION BY CALMODULIN AND A CALMODULIN-LIKE PROTEIN ISOLATED FROM SYNAPTIC VESICLES
STIMULATION OF CA2+-DEPENDENT NEUROTRANSMITTER RELEASE AND PRESYNAPTIC NERVE-TERMINAL PROTEIN-PHOSPHORYLATION BY CALMODULIN AND A CALMODULIN-LIKE PROTEIN ISOLATED FROM SYNAPTIC VESICLES
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DOI：
10.1073/pnas.76.4.1838
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
MAURER, SC
中科院分区：
文献类型：
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作者：
DELORENZO, RJ;FREEDMAN, SD;MAURER, SC
突触囊泡具有Ca2+依赖性蛋白激酶系统，其可能在介导Ca2+刺激的神经递质释放和囊泡功能中发挥作用。Ca2+的能力，启动去甲肾上腺素释放和蛋白磷酸化突触囊泡制剂刺激的内源性热稳定囊泡蛋白组分的存在下。这种内源性囊泡组分的热稳定性和特性与从大鼠和牛脑中分离的钙调蛋白（Ca2+依赖的常规蛋白）的热稳定性和特性相似。钙调素，像内源性热稳定囊泡因子，恢复刺激囊泡神经递质释放和蛋白激酶活性的能力。钙调素样囊泡蛋白和纯化的钙调素在刺激环核苷酸依赖性磷酸二酯酶中同样有效，进一步表明这2种蛋白质在功能上是等同的。在完整的突触体中，去极化依赖的Ca 2+摄取同时刺激神经递质的释放和特定突触囊泡蛋白的磷酸化，这些蛋白在分离的囊泡制备物中显示依赖于Ca 2+和钙调蛋白。钙对神经递质释放和突触前神经终末蛋白磷酸化的影响可能是通过内源性钙调素样蛋白介导的。
Synaptic vesicles have a Ca2+-dependent protein kinase system that may play a role in mediating Ca2+-stimulated neurotransmitter release and vesicle function. The ability of Ca2+ to initiate norepinephrine release and protein phosphorylation in synaptic vesicle preparations was stimulated by the presence of an endogenous heat-stable vesicle protein fraction. The heat stability and characteristics of this endogenous vesicle fraction were similar to those of calmodulin (Ca2+-dependent regular protein) isolated from rat and bovine brain. Calmodulin, like endogenous heat-stable vesicle factor, restored the ability to stimulate vesicle neurotransmitter release and protein kinase activity. Calmodulin-like vesicle protein and purified calmodulin were equally effective in stimulating cyclic nucleotide-dependent phosphodiesterase, further indicating that these 2 proteins are functionally equivalent. Depolarization-dependent Ca2+ uptake in intact synaptosomes simultaneously stimulated release of neurotransmitter and phosphorylationof particular synaptic vesicle proteins that were shown in the isolated vesicle preparation to be dependent on Ca2+ and calmodulin. The effects of Ca on neurotransmitter release and presynaptic nerve terminal protein phosphorylation may be mediated by endogenous calmodulin-like proteins.