Vertical distribution of antibiotic resistance genes in an urban green facade.
Vertical distribution of antibiotic resistance genes in an urban green facade.
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DOI：
10.1016/j.envint.2021.106502
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发表时间：
2021-03
影响因子：
11.8
通讯作者：
Shuyidan Zhou;Qi Zhang;R. Neilson;M. Giles;Hu Li;Xiao-Ru Yang;Jianqiang Su;Yong-guan Zhu
中科院分区：
文献类型：
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作者：
Shuyidan Zhou;Qi Zhang;R. Neilson;M. Giles;Hu Li;Xiao-Ru Yang;Jianqiang Su;Yong-guan Zhu
叶球被认为是一个关键的网站，为转移的天然和人工选择的抗微生物药物耐药基因（ARG）到人类。因此，绿色建筑系统的发展可能会为ARG和病原体从环境转移到室外植物提供一条尚未探索的途径。我们收集了从植物爬上建筑物在1，2，4和15米以上的地面水平和收集相关的灰尘样品从相邻的窗台，以确定微生物群和ARG的多样性和相对丰度。总体而言，共检测到来自11个主要类别的143个ARG和18个移动的遗传元件（MGE）。叶际ARGs的相对丰度随离地高度的增加而降低。快速期望最大化微生物源追踪（FEAST）表明，土壤和气溶胶对叶际微生物的贡献是有限的。从植物组织中分离细菌的培养依赖性方法从根、茎和叶样品以及从叶中分离的内生菌中鉴定出总共91个属。在这些细菌中，代表9个属的20个分离株是已知的人类致病成员。来自培养依赖性和培养非依赖性方法的共有细菌表明，微生物可能通过内生机制从土壤移动到植物，因此，ARG和人类病原体从户外环境中移动的可能性很明显。
The phyllosphere is considered a key site for the transfer of both naturally and anthropogenically selected antimicrobial resistance genes (ARGs) to humans. Consequently, the development of green building systems may pose an, as yet, unexplored pathway for ARGs and pathogens to transfer from the environment to outdoor plants. We collected leaves from plants climbing up buildings at 1, 2, 4 and 15 m above ground level and collected associated dust samples from adjacent windowsills to determine the diversity and relative abundance of microbiota and ARGs. Overall, a total of 143 ARGs from 11 major classes and 18 mobile genetic elements (MGEs) were detected. The relative abundance of ARGs within the phyllosphere decreased with increasing height above ground level. Fast expectation–maximization microbial source tracking (FEAST) suggested that the contribution of soil and aerosols to the phyllosphere microbiome was limited. A culture-dependent method to isolate bacteria from plant tissues identified a total of 91 genera from root, stem, and leaf samples as well as endophytes isolated from leaves. Of those bacteria, 20 isolates representing 9 genera were known human pathogenic members to humans. Shared bacterial from culture-dependent and culture-independent methods suggest microorganisms may move from soil to plant, potentially through an endophytic mechanism and thus, there is a clear potential for movement of ARGs and human pathogens from the outdoor environment.