Metagenomic Analysis of 0.2-μm-Passable Microorganisms in Deep-Sea Hydrothermal Fluid
Metagenomic Analysis of 0.2-μm-Passable Microorganisms in Deep-Sea Hydrothermal Fluid
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DOI：
10.1007/s10126-010-9351-6
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发表时间：
2011-10-01
影响因子：
3
通讯作者：
Naganuma, Takeshi
中科院分区：
文献类型：
--
作者：
Nakai, Ryosuke;Abe, Takashi;Naganuma, Takeshi
我们对从微生物中提取的大量DNA进行了焦解测序，这些微生物通过0.2微米孔径的过滤器，并被0.1微米孔径过滤器捕获在马里亚纳海槽的热水流体中。使用454flx测序仪，我们产生了202,648个序列，平均长度为173.8个碱基。功能配置文件由种子注释引擎分配。在0.2亩传代微生物的元基因组中，与膜功能有关的基因占主导地位，包括被归类为膜转运的钾稳态和被归类为毒力的多药耐药外排泵。在我们的超基因组文库中，与膜转运相关的基因比例高于其他大洋和热液喷口的超基因组。与RND型外源物质外排转运体相关的基因在本研究中得到了明确的鉴定。在与已知的超微细菌Sphingopyxis alaskensis RB2256的基因组进行比较分析后，我们在我们的超基因组中发现了1,542个显著的HITS(E<；1×10(-2))，其中1,172个与DNA修复蛋白RADA有关。在这种情况下，本研究中0.2微米可通过组分的微生物功能谱不同于海洋中的0.2微米捕获组分和前人报道的热液喷口组分。
We pyrosequenced the bulk DNA extracted from microorganisms that passed through 0.2-mu m-pore-size filters and trapped by 0.1-mu m-pore-size filters in the hydrothermal fluid of the Mariana Trough. Using the 454-FLX sequencer, we generated 202,648 sequences with an average length of 173.8 bases. Functional profiles were assigned by the SEED Annotation Engine. In the metagenome of the 0.2-mu m-passable microorganisms, genes related to membrane function, including potassium homeostasis classified as membrane transport, and multidrug-resistance efflux pumps classified as virulence, were dominant. There was a higher proportion of genes pertinent to the subsystem of membrane transport in our metagenomic library than in other oceanic and hydrothermal vent metagenomes. Genes associated with a RND-type efflux transporter for exogenous substances were specifically identified in the present study. After a comparative analysis with the genome of the known ultramicrobacterium Sphingopyxis alaskensis RB2256, we discovered 1,542 cases of significant hits (E < 1 x 10(-2)) in our metagenome, and 1,172 of those were related to the DNA repair protein RadA. In this way, the microbial functional profile of 0.2-mu m-passable fraction in the present study differs from oceanic metagenomes in the 0.2-mu m-trapped fractions and hydrothermal vent metagenomes reported in previous research.