Topically applied fatty acids are elongated before incorporation in the stratum corneum lipid matrix in compromised skin
Topically applied fatty acids are elongated before incorporation in the stratum corneum lipid matrix in compromised skin
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DOI：
10.1111/exd.13116
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发表时间：
2017-01-01
影响因子：
3.6
通讯作者：
Bouwstra, Joke A.
中科院分区：
文献类型：
--
作者：
Berkers, Tineke;van Dijk, Lauri;Bouwstra, Joke A.
在一些皮肤病中，角质层（SC）的脂质组成和组织都发生了改变，从而导致患者皮肤屏障功能受损。皮肤屏障修复的方法之一是局部配方治疗，使SC脂质组成和组织正常化。妊娠期涂抹在皮肤上的白色干酪乳霜（VC）已被证明能增强皮肤屏障修复。在这项研究中，我们研究了一种含有脂肪酸（FA）的制剂如何模拟VC的脂质组成与SC中的脂质基质相互作用。该制剂在SC去除后应用于离体人体皮肤。随后，离体人皮肤在培养过程中产生SC。采用傅里叶变换红外光谱（FTIR）和液相色谱质谱（LC/MS）分析了含FA制剂对再生SC中脂质组织和组成的影响。FTIR结果表明，FAs嵌入到再生SC的脂质基质中，并与内源性SC脂质在同一晶格中划分，从而增加了SC中形成正交（非常致密）填料的脂质比例。LC/MS数据表明，局部应用的FAs在嵌入脂质基质之前被延长，因此参与了皮肤中的脂质生物合成。
In several skin diseases, both the lipid composition and organization in the stratum corneum (SC) are altered which contributes to the impaired skin barrier function in patients. One of the approaches for skin barrier repair is treatment with topical formulations to normalize SC lipid composition and organization. Vernix caseosa (VC), a white cheesy cream on the skin during gestational delivery, has shown to enhance skin barrier repair. In this study, we examined how a fatty acid (FA) containing formulation mimicking the lipid composition of VC interacts with the lipid matrix in the SC. The formulation was applied on ex vivo human skin after SC removal. Subsequently, the ex vivo human skin generated SC during culture. The effect of FA containing formulations on the lipid organization and composition in the regenerated SC was analysed by Fourier transform infrared (FTIR) spectroscopy and liquid chromatography mass spectroscopy (LC/MS), respectively. FTIR results demonstrate that the FAs are intercalated in the lipid matrix of the regenerated SC and partition in the same lattice with the endogenous SC lipids, thereby enhancing the fraction of lipids forming an orthorhombic (very dense) packing in the SC. LC/MS data show that the topically applied FAs are elongated before intercalation in the lipid matrix and are thus involved in the lipid biosynthesis in the skin.