Effect of (in)organic additives on microbially induced calcium carbonate precipitation
Effect of (in)organic additives on microbially induced calcium carbonate precipitation
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DOI：
10.1093/jambio/lxad309
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发表时间：
2024-01-30
影响因子：
4
通讯作者：
Zhang，Meng
中科院分区：
文献类型：
--
作者：
Haystead，Jamie;Gilmour，Katie;Zhang，Meng
目的本研究旨在了解（内）有机添加剂对微生物诱导碳酸钙沉淀（MICP）的形态影响。方法和结果在（内）有机添加剂存在下实时监测MICP：牛血清白蛋白（BSA）、生物膜表面层蛋白A（BslA）、氯化镁（MgCl2）和聚-L-赖氨酸。该监测是使用共焦显微镜进行的，以观察从成核点开始的 CaCO3 的形成，并与没有添加剂的条件进行比较。补充方法，即扫描电子显微镜、能量色散 X 射线光谱和 X 射线衍射，分别用于评估晶体形成后 CaCO3 的视觉形态、元素组成和晶体结构。结果表明，与没有添加剂的反应相比，在存在添加剂的情况下，100 分钟时产生了更多的 CaCO3 晶体。与含有其他添加剂（包括 MgCl2）的反应相比，添加 BslA 会产生更大的晶体。 BSA 在反应的早期阶段（20 分钟）诱导产生大量晶体，但与无添加剂的条件相比，对晶体尺寸没有显着影响。反应 24 小时后，与球霰石相比，所有添加剂都产生了更高的方解石含量，但 MgCl2 除外，它产生了大量的镁方解石。结论该工作证明了几种（无机）有机添加剂对 MICP 的影响，并为进一步研究了解添加剂对 MICP 的影响以实现受控 CaCO3 沉淀奠定了基础。
AimThis study aimed to understand the morphological effects of (in)organic additives on microbially induced calcium carbonate precipitation (MICP).Methods and resultsMICP was monitored in real time in the presence of (in)organic additives: bovine serum albumin (BSA), biofilm surface layer protein A (BslA), magnesium chloride (MgCl2), and poly-l-lysine. This monitoring was carried out using confocal microscopy to observe the formation of CaCO3from the point of nucleation, in comparison to conditions without additives. Complementary methodologies, namely scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction, were employed to assess the visual morphology, elemental composition, and crystalline structures of CaCO3, respectively, following the crystals’ formation. The results demonstrated that in the presence of additives, more CaCO3crystals were produced at 100 min compared to the reaction without additives. The inclusion of BslA resulted in larger crystals than reactions containing other additives, including MgCl2. BSA induced a significant number of crystals from the early stages of the reaction (20 min) but did not have a substantial impact on crystal size compared to conditions without additives. All additives led to a higher content of calcite compared to vaterite after a 24-h reaction, with the exception of MgCl2, which produced a substantial quantity of magnesium calcite.ConclusionsThe work demonstrates the effect of several (in)organic additives on MICP and sets the stage for further research to understand additive effects on MICP to achieve controlled CaCO3precipitation.