Stretching, unfolding, and deforming protein filaments adsorbed at solid-liquid interfaces using the tip of an atomic-force microscope.
Stretching, unfolding, and deforming protein filaments adsorbed at solid-liquid interfaces using the tip of an atomic-force microscope.
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使用原子力显微镜的尖端拉伸、展开和变形吸附在固液界面上的蛋白质丝。
DOI：
10.1103/physrevlett.102.128302
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发表时间：
2009
影响因子：
8.6
通讯作者：
L. Kreplak
中科院分区：
文献类型：
--
作者：
Douglas B. Staple;M. Loparić;H. Kreuzer;L. Kreplak
细胞通过主动重塑密集的蛋白丝网络来移动。在这里，我们在简化的实验装置中分析了各种细丝的力响应，其中使用原子力显微镜 (AFM) 尖端移动单根细丝以克服表面摩擦，并且 AFM 在扭转模式下运行。我们的实验结果在基于牛顿力学的简单模型中得到了很好的解释：我们观察到力平台，这是单个重复单元顺序拉伸的特征，最终导致整个聚合物形状变形。
Cells move by actively remodeling a dense network of protein filaments. Here we analyze the force response of various filaments in a simplified experimental setup, where single filaments are moved with an atomic-force microscope (AFM) tip against surface friction, with the AFM operating in the torsional mode. Our experimental findings are well explained within a simple model based on Newtonian mechanics: we observe force plateaus, which are the signature of the sequential stretching of single repeat units, followed ultimately by deformation of the whole polymer shape.