Modulation of neuronal activity in cortical organoids with bioelectronic delivery of ions and neurotransmitters.
Modulation of neuronal activity in cortical organoids with bioelectronic delivery of ions and neurotransmitters.
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通过离子和神经递质的生物电子传递来调节皮质类器官中的神经元活动。
DOI：
10.1101/2023.06.10.544416
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Aslankoohi,Elham
中科院分区：
文献类型：
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作者：
Park,Yunjeong;Hernandez,Sebastian;Hernandez,CristianO;Schweiger,HunterE;Li,Houpu;Voitiuk,Kateryna;Dechiraju,Harika;Hawthorne,Nico;Muzzy,ElanaM;Selberg,JohnA;Sullivan,FrederikaN;Urcuyo,Roberto;Salama,SofieR;Aslankoohi,Elham
大脑活动的精确调节对于大脑皮层的正确建立和成熟是至关重要的。为此，皮质类器官是研究回路形成和神经发育疾病基础的有前途的工具。然而，在脑类器官中以高时间分辨率操纵神经元活动的能力仍然有限。为了克服这一挑战，我们引入了一种生物电子的方法来控制皮质类器官的活动与离子和神经递质的选择性交付。使用这种方法，我们分别通过钾离子（K+）和γ-氨基丁酸（GABA）的生物电子传递依次增加和减少脑类器官中的神经元活动，同时监测网络活动。这项工作突出了生物电子离子泵作为工具，用于高分辨率的时间控制大脑类器官的活动，以精确的药理学研究，可以提高我们对神经元功能的理解。
Precise modulation of brain activity is fundamental for the proper establishment and maturation of the cerebral cortex. To this end, cortical organoids are promising tools to study circuit formation and the underpinnings of neurodevelopmental disease. However, the ability to manipulate neuronal activity with high temporal resolution in brain organoids remains limited. To overcome this challenge, we introduce a bioelectronic approach to control cortical organoid activity with the selective delivery of ions and neurotransmitters. Using this approach, we sequentially increased and decreased neuronal activity in brain organoids with the bioelectronic delivery of potassium ions (K+) and γ-aminobutyric acid (GABA), respectively, while simultaneously monitoring network activity. This works highlights bioelectronic ion pumps as tools for high-resolution temporal control of brain organoid activity toward precise pharmacological studies that can improve our understanding of neuronal function.