OSMOTIC FLOW CAUSED BY CHONDROITIN SULFATE PROTEOGLYCAN ACROSS WELL-DEFINED NUCLEOPORE MEMBRANES
OSMOTIC FLOW CAUSED BY CHONDROITIN SULFATE PROTEOGLYCAN ACROSS WELL-DEFINED NUCLEOPORE MEMBRANES
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DOI：
10.1016/0301-4622(90)80027-5
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发表时间：
1990-08-15
影响因子：
3.8
通讯作者：
WILLIAMS, RPW
中科院分区：
文献类型：
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作者：
COMPER, WD;WILLIAMS, RPW
利用已知半径Rp的明确直通圆柱孔的核孔膜，分析了大鼠软骨肉瘤蛋白多糖亚基溶液产生的渗透流。研究了Rp在27-500 nm范围内的膜。对于不能透过蛋白多糖的半透膜，流动始终与RP2相关，而不是RP4(泊松定律)，这表明该流动是如前所述的扩散控制过程(R.P.W.Williams和W.D.Comper，J.Phys)。化学。91(1987)3443)。我们还确定了一个特征距离，大约。平均颗粒间距的50%，从膜的孔表面出来，蛋白多糖必须在其上移动才能产生流动。蛋白多糖产生的渗透系数与膜的渗透系数相似，孔径最大可达125 nm，显着大于平均分子间距离。这些结果被解释为膜孔识别蛋白多糖溶液中动态的瞬时聚集体。
Osmotic flows generated by solutions of Swarm rat chondrosarcoma proteoglycan subunit have been analysed using Nuclepore membranes of well-defined straight-through cylindrical pores of known radius rp. Membranes with rp in the range of 27-500 nm were studied. For semipermeable membranes, which are impermeable to the proteoglycan, the flows were consistently related to rp2 and not to rp4 (Poiseuille''s Law) which demonstrates that the flow is a diffusion-controlled process as described previously (R.P.W. Williams and W.D. Comper, J. Phys. Chem. 91 (1987) 3443). We have also identified a characteristic distance, approx. 50% of the average interparticle spacing, out from the pore surface of the membrane over which the proteoglycan has to move to generate flow. The proteoglycan generates similar osmotic permeability coefficients with membranes up to 125 nm in pore diameter which is significantly larger than the average intermolecular distance. These results have been interpreted in terms of the membrane pore recognising dynamic transient aggregates in the proteoglycan solution.