Maternal transmission of immunity to white spot syndrome associated virus (WSSV) in shrimp (Penaeus monodon)
Maternal transmission of immunity to white spot syndrome associated virus (WSSV) in shrimp (Penaeus monodon)
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DOI：
10.1016/s0145-305x(99)00038-5
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发表时间：
1999-10-01
影响因子：
2.9
通讯作者：
Song, YL
中科院分区：
文献类型：
--
作者：
Huang, CC;Song, YL
在本研究中使用了从面包酵母酿酒酵母中提取的β-1，3-1，6-葡聚糖，以研究葡聚糖能够在多大程度上保护产卵鱼免受白色斑点综合征相关病毒（WSSV）的侵害，以及这种保护（如果有的话）是否可以通过母体免疫传递给孵化的幼鱼。结果表明，在眼柄切除后至重复产卵结束的15 d内，注射葡聚糖组中出现体色变红和壳上出现白色斑点等临床症状的产卵鱼较少。这表明，葡聚糖的应用可能会导致产卵鱼抗病性的轻微增强，尽管在选定的置信限度内，差异在统计学上并不显著。挑战结果显示，与来自未处理产卵器组的幼虫相比，来自葡聚糖注射产卵器组的幼虫的相对存活百分比显著增加。因此，似乎葡聚糖诱导的母体传播疾病抗性保护了幼虫免受WSSV感染。葡聚糖浸泡不仅显示出对来自未注射葡聚糖的产卵体的无节幼体有效，它还为已经具有对WSSV的母系传播抗性的无节幼体提供了额外的累积保护。这是第一次有文献证明无脊椎动物的免疫力是通过母体传播的。(C)1999爱思唯尔科技有限公司。保留所有权利。
beta-1,3-1,6-glucan, derived from bakers' yeast Saccharomyces cerevisiae, was used in the present study to investigate the extent to which glucan is able to protect spawners from white spot syndrome associated virus (WSSV), and whether this protection (if any) can be passed on to hatchlings via maternal transmission of immunity. Results showed that fewer spawners in the glucan-injected groups showed the clinical symptoms of red body coloration and white spots on the shell during the 15 days between eyestalk ablation and the end of repeated spawning. This suggests that the application of glucan might lead to a slight enhancement of disease resistance in spawners, although the differences were not statistically significant within the confidence limit chosen. Challenge results showed a significant increase in relative percent survival for larvae derived from groups of glucan-injected spawners compared to those derived from groups of untreated spawners. It therefore seems that a maternally transmitted disease resistance induced by glucan, protected the larvae against a WSSV infection. Glucan immersion was not only shown to be effective for nauplii derived from spawners that were not injected with glucan, it also provided additional, cumulative protection for nauplii which already had a maternally transmitted resistance to WSSV. This is the first documented demonstration of a maternal transmission of immunity in invertebrates. (C) 1999 Elsevier Science Ltd. All rights reserved.