Role of antigen size and charge in immune complex glomerulonephritis.
Role of antigen size and charge in immune complex glomerulonephritis.
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抗原大小和电荷在免疫复合物肾小球肾炎中的作用。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
F. Miller
中科院分区：
文献类型：
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作者：
K. Isaacs;F. Miller
为了探索大小和电荷在免疫复合物肾小球肾炎中的作用，通过添加带电基团（二乙基氨基乙基葡聚糖、中性葡聚糖、硫酸葡聚糖）来修饰一系列大小（10，000-、70，000-、500，000-道尔顿）的葡聚糖，并将其施用给小鼠。动物发生肾小球肾炎，其特征为伊加沉积，IgM和C3含量较少。肾小球反应的模式是电荷依赖性的，中性抗原引起局灶性节段性增殖反应，系膜沉积量很少。阴离子硫酸葡聚糖导致弥漫性系膜增生图片与重沉积。阳离子，二乙基氨基乙基葡聚糖治疗的动物表现出显着增加系膜基质少得多的增殖。该模型对复杂沉积机制的重要性，即，循环与原位，和多糖抗原的潜在重要性（这项研究代表了这类物质的肾炎的第一个积极诱导）进行了讨论。
To explore the role of size and charge in immune complex glomerulonephritis, a series of sized (10,000-, 70,000-, 500,000-dalton) dextrans was modified by the addition of charged groups (diethylaminoethyl dextran, neutral dextran, dextran sulfate) and administered to mice. The animals developed glomerulonephritis characterized by the deposition of IgA with lesser amounts of IgM and C3. The pattern of glomerular response was charge dependent with neutral antigens eliciting a focal, segmental proliferative response with mesangial deposition in scant amounts. The anionic dextran sulfate led to a diffuse mesangial proliferative picture with heavy deposition. The cationic, diethylaminoethyl-dextran-treated animals showed a marked increase in mesangial matrix with much less proliferation. The importance of this model to mechanisms of complex deposition, i.e., circulating versus in situ, and the potential importance of polysaccharide antigens (this study representing the first active induction of nephritis with this class of substances) is discussed.