Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test
Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test
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DOI：
10.1038/s41598-019-51342-2
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发表时间：
2019-10-14
影响因子：
4.6
通讯作者：
Muro, A.
中科院分区：
文献类型：
--
作者：
Garcia-Bernalt Diego, J.;Fernandez-Soto, P.;Muro, A.
血吸虫病是最普遍的被忽视的热带疾病之一，影响全世界约2.5亿人。曼氏血吸虫是引起人类肠道血吸虫病的最重要的虫种。尽管近几十年来做出了重大努力，但血吸虫病的全球疾病负担仍然非常高。部分原因可能是缺乏准确的诊断工具，主要是在流行地区。环介导的等温扩增（LAMP）作为许多其他复杂分子方法的现场友好的替代方案越来越多地用于分子诊断，并且它已被提出作为革命性的床旁分子诊断的理想候选者。在以前的工作中，基于LAMP的方法来检测S。本课题组研制了一种用于早期诊断活动性血吸虫病的曼氏DNA（SmMIT-LAMP）。SmMIT-LAMP已在人类粪便和蜗牛样本中进一步成功评估，最近，在人类尿液样本中。在这项研究中，我们开发了一个重要的改进SmMIT-LAMP分子检测，将其转化为一个冷保持干燥的格式适合于潜在的生产，即用型血吸虫病诊断试剂盒。这一程序可用于制作用于实验室环境和用于其他被忽视的热带疾病的诊断应用的干LAMP试剂盒。
Schistosomiasis is one of the most prevalent Neglected Tropical Disease, affecting approximately 250 million people worldwide. Schistosoma mansoni is the most important species causing human intestinal schistosomiasis. Despite significant efforts in recent decades, the global disease burden of schistosomiasis remains extremely high. This could partly be attributed to the absence of accurate diagnostic tools, primarily in endemic areas. Loop-mediated isothermal amplification (LAMP) is increasingly used in molecular diagnostics as a field-friendly alternative to many other complex molecular methods and it has been proposed as an ideal candidate for revolutionizing point-of-care molecular diagnostics. In a previous work, a LAMP-based method to detect S. mansoni DNA (SmMIT-LAMP) was developed by our research group for early diagnosis of active schistosomiasis in an experimental infection murine model. The SmMIT-LAMP has been further successfully evaluated in both human stool and snail samples and, recently, in human urine samples. In this study, we developed an important improvement for SmMIT-LAMP molecular assay, transforming it into a cold maintenance dry format suitable for potentially manufacturing as kit for ready-to-use for schistosomiasis diagnosis. This procedure could be applied to create dry LAMP kits for a laboratory setting and for diagnostic applications for other neglected tropical diseases.