THE BACTERIOLOGY AND CYTOLOGY OF CHRONIC OTITIS-MEDIA WITH EFFUSION
THE BACTERIOLOGY AND CYTOLOGY OF CHRONIC OTITIS-MEDIA WITH EFFUSION
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DOI：
10.1097/00006454-198203000-00007
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发表时间：
1982-01-01
影响因子：
3.6
通讯作者：
LE, CT
中科院分区：
文献类型：
--
作者：
GIEBINK, GS;JUHN, SK;LE, CT
对729例持续性分泌性中耳炎患儿中耳积液进行细菌学和细胞学检查。35%的慢性渗出液培养阳性。B型和非B型流感嗜血杆菌、肺炎链球菌、奈瑟菌属和表皮葡萄球菌是主要分离株。浆液性和粘液性积液培养产生的细菌往往在年轻比年龄较大的儿童。此外，在17%的无菌积液的革兰氏染色涂片中观察到细菌;革兰氏阳性球菌在这些积液中占主导地位。32%的双侧中耳炎病例获得了不同的渗出液培养结果。渗出液产生H。流感和S. pneumoniae培养的多形核白细胞比产生S.表皮葡萄球菌或奈瑟氏球菌或不育。在革兰氏染色上，吞噬细胞在有或无细菌的无菌渗出液中同样普遍。与未经治疗的患者相比，在接受抗肿瘤治疗的患者的粘液样渗出液中较少见到吞噬细胞。结果表明，慢性中耳积液中的某些细菌通过引发局部炎性细胞反应而促成了这种情况的发病机制。
The bacteriology and cytology of middle ear effusion from 729 children with persistent otitis media with effusion were studied. Thirty-five percent of these chronic effusions were culture-positive. Type b and non-type b Haemophilus influenzae, Streptococcus pneumoniae, Neisseria sp. and Staphylococcus epidermidis were the predominant isolates. Serous and mucoid effusion cultures yielded bacteria more often in younger than in older children. In addition bacteria were seen in 17% of the Gram-stained smears of the sterile effusions; Gram-positive cocci predominated in these effusions. Disparate effusion culture results were obtained in 32% of bilateral otitis media cases. Effusions which yielded H. influenzae and S. pneumoniae on culture had more polymorphonuclear leukocytes than did effusions which yielded S. epidermidis or Neisseria or were sterile. Phagocytic cells were equally prevalent in sterile effusions with or without bacteria on Gram stain. Phagocytic cells were seen less often in mucoid effusions from antibiotic-treated patients than in mucoid effusions from untreated patients. The results suggest that certain bacteria in chronic middle ear effusion contribute to the pathogenesis of this condition by eliciting a local inflammatory cell response.