The selection of S. cerevisiae mutants defective in the start event of cell division.
The selection of S. cerevisiae mutants defective in the start event of cell division.
复制标题
选择细胞分裂起始事件有缺陷的酿酒酵母突变体。
DOI：
10.1093/genetics/95.3.561
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发表时间：
1980
期刊：
影响因子：
3.3
通讯作者：
Reed,SI
中科院分区：
文献类型：
--
作者：
Reed,SI
分离了酿酒酵母细胞分裂周期起始事件中存在缺陷的 33 个温度敏感突变，并进行了初步表征。互补研究将这些突变分配给四个互补组，其中之一，cdc28，之前已描述过。遗传分析表明，这些互补组定义了单个核基因，彼此不相关。三个新发现的基因之一，cdc37，位于第四号染色体上的酵母连锁图中，即距hom2远端的两个减数分裂图单位。每个突变都会在细胞分裂开始时产生阶段特异性的停滞，这也是交配信息素中断分裂的点。通过在限制性温度下孵育开始同步化后，突变体保留了扩增和缀合的能力。
Thirty-three temperature-sensitive mutations defective in the start event of the cell division cycle ofSaccharomyces cereuisiaewere isolated and subjected to preliminary characterization. Complementation studies assigned these mutations to four complementation groups, one of which,cdc28, has been described previously. Genetic analysis revealed that these complementation groups define single nuclear genes, unlinked to one another. One of the three newly identified genes,cdc37, has been located in the yeast linkage map on chromosomeIV, two meiotic map units distal tohom2.—Each mutation produces stage-specific arrest of cell division at start, the same point where mating pheromone interrupts division. After synchronization at start by incubation at the restrictive temperature, the mutants retain the capacity to enlarge and to conjugate.