Semaphorin 3A expression following intestinal ischemia/reperfusion injury in Sox10-Venus mice.
Semaphorin 3A expression following intestinal ischemia/reperfusion injury in Sox10-Venus mice.
复制标题
Sox10-Venus 小鼠肠道缺血/再灌注损伤后信号蛋白 3A 的表达。
DOI：
10.1007/s00383-016-4039-2
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yamataka A.
中科院分区：
文献类型：
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作者：
Takeda M;Miyahara K;Okawada M;Akazawa C;Lane GJ;Yamataka A.
脑信号蛋白3A（Semaphorin 3A，Sema 3A）是神经系统发育过程中分泌的一种蛋白质，在神经元的病理生理中起重要作用。然而，Sema 3A和肠缺血/再灌注（I/R）损伤之间没有已知的相关性。我们评估了Sema 3A的表达和分布与肠神经系统（ENS）损伤后看到的肠I/R injury in Sox 10-Venus mice.Methodsintestinal I/R injury was induced by vascular occlusion for 3 h.再灌注后0、3、12、24、48和96 h采集回肠标本。立体显微镜和荧光显微镜被用来评估sox 10-Venus+细胞和PGP9.5+cells.ResultsBy 3 h后再灌注，Sema 3A的表达增加到最大和sox 10-Venus+细胞已经褪色到最低限度收获回肠段。两种差异均具有统计学显著性。到再灌注后96小时，Sema 3A和Sox 10-Venus+细胞荧光均恢复到原始水平。苏木素和伊红染色确定模仿Sema 3A表达的组织损伤，而PGP9.5+细胞response was minimal.ConclusionWe是第一个证明Sema 3A表达和ENS损伤之间的相关性，在Sox 10-Venus小鼠肠I/R。
PurposeSemaphorin 3A (Sema3A) is a protein secreted during development of the nervous system that plays an important role in neuronal pathophysiology. However, there is no known correlation between Sema3A and intestinal ischemia/reperfusion (I/R) injury. We assessed Sema3A expression and distribution in relation to enteric nervous system (ENS) damage seen after intestinal I/R injury in Sox10-Venus mice.MethodsIntestinal I/R injury was induced by vascular occlusion for 3 h. Ileal specimens were harvested 0, 3, 12, 24, 48, and 96 h after reperfusion. Stereoscopic microscopy and fluorescence microscopy were used to assess sox10-Venus+cells and PGP9.5+cells.ResultsBy 3 h after reperfusion, Sema3A expression had increased to a maximum and Sox10-Venus+cells had faded to a minimum in harvested ileal segments. Both differences were statistically significant. By 96 h after reperfusion, both Sema3A and Sox10-Venus+cell fluorescence had reverted to original levels. Hematoxylin and eosin staining identified histologic damage mimicking Sema3A expression, while PGP9.5+cell response was minimal.ConclusionWe are the first to demonstrate a correlation between Sema3A expression and ENS damage following intestinal I/R in Sox10-Venus mice.