Expression of Migration-Related Genes in Human Colorectal Cancer and Activity of a Disintegrin and Metalloproteinase 17.
Expression of Migration-Related Genes in Human Colorectal Cancer and Activity of a Disintegrin and Metalloproteinase 17.
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DOI：
10.1155/2016/8208904
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发表时间：
2016
影响因子：
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通讯作者：
Muc-Wierzgoń M
中科院分区：
文献类型：
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作者：
Walkiewicz K;Kozieł P;Bednarczyk M;Błażelonis A;Mazurek U;Muc-Wierzgoń M
导论.依赖于细胞迁移机制的形成转移的能力是癌症进展的重要因素。在本研究中，我们分析了参与结肠癌细胞迁移调控的基因。材料和方法。分析了来自临床阶段I-II和III-IV的结直肠癌患者的总共20对手术切除的肿瘤和健康（边缘）组织样品。根据制造商的说明书进行来自CRC和正常组织的RNA的分离及其随后的分子分析。使用GeneSpring 11.5平台和微阵列显著性分析（SAM）进行微阵列数据分析。在SAM分析中，使用评分和q值参数来识别显着差异表达的基因。结果MMP 9、ADAM 17、EphA 2和TIMP显示基因表达的最大增加。结论.所呈现的基因，特别是ADAM 17、MMP 9、EphA 2、TIMP 1、ICAM 11和CD 4，可用作晚期结直肠癌的预后标志物，有助于开发专注于减少原发性肿瘤转移的新疗法。
Introduction. The ability to form metastases which depends on the mechanisms of cell migration is an important element of the progression of cancer. In the present study we analyzed the genes involved in the regulation of migration in colon cancer cells. Materials and Methods. A total of 20 pairs of surgically removed tumoral and healthy (marginal) tissues samples from colorectal cancer patients at clinical stages I-II and III-IV were analyzed. The isolation of RNA from CRC and normal tissues and its subsequent molecular analysis were performed according to manufacturer's instructions. Microarray data analysis was performed using the GeneSpring 11.5 platform and Significance Analysis of Microarrays (SAM). In SAM analysis to identify significantly differentially expressed genes score and q-value parameters were used. Results. The largest increase in expression of genes was shown by MMP9, ADAM17, EphA2, and TIMP. Conclusions. Presented genes, especially ADAM17, MMP9, EphA2, TIMP1, ICAM 11, and CD4, may be used as prognostic markers of advanced stages of colorectal cancer, contributing to the development of new lines of therapy focused on reducing metastasis of the primary tumor.