Attachment of and biofilm formation by Enterobacter sakazakii on stainless steel and enteral feeding tubes
Attachment of and biofilm formation by Enterobacter sakazakii on stainless steel and enteral feeding tubes
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DOI：
10.1128/aem.00654-06
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发表时间：
2006-09-01
影响因子：
4.4
通讯作者：
Beuchat, Larry R.
中科院分区：
文献类型：
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作者：
Kim, Hoikyung;Ryu, Jee-Hoon;Beuchat, Larry R.
据报道坂崎肠杆菌会形成生物膜，但影响非生物表面附着和生物膜形成的环境条件尚未描述。本研究探讨了温度和营养盐对E.不锈钢和肠内营养管上的坂崎。在胰蛋白酶大豆肉汤（TSB），婴儿配方肉汤（IFB），或生菜汁肉汤（LJB）在12和25摄氏度生长到稳定期的五个菌株进行了检查，在何种程度上，他们重视这些材料。在25 ℃时附着的种群比在12 ℃时附着的种群多。将不锈钢试样和肠饲管在4 ℃下浸入磷酸盐缓冲盐水悬浮液（7 log CFU/ml）中24 h，以分别促进5.33至5.51和5.03至5.12 log CFU/cm（2）的附着，然后浸入TSB、IFB或LJB中，随后在12或25 ℃下孵育10天。生物膜在12 ℃下不产生。在25 ℃的IFB中，不锈钢管和饲管上形成的生物膜中试验菌株的细胞数分别增加了1.42 ~ 1.67 log CFU/cm（2）和1.16 ~ 1.31 log CFU/cm（2）;在25 ℃下在TSB和LJB上没有形成生物膜，这表明养分的可利用性在导致这些惰性材料表面上的生物膜形成的过程中起主要作用。这些观察结果强调了复溶婴儿配方奶粉制备和储存区域的温度控制对于防止大肠杆菌附着和生物膜形成的重要性。坂崎。
Enterobacter sakazakii has been reported to form biofilms, but environmental conditions affecting attachment to and biofilm formation on abiotic surfaces have not been described. We did a study to determine the effects of temperature and nutrient availability on attachment and biofilm formation by E. sakazakii on stainless steel and enteral feeding tubes. Five strains grown to stationary phase in tryptic soy broth (TSB), infant formula broth (IFB), or lettuce juice broth (LJB) at 12 and 25 degrees C were examined for the extent to which they attach to these materials. Higher populations attached at 25 degrees C than at 12 degrees C. Stainless steel coupons and enteral feeding tubes were immersed for 24 h at 4 degrees C in phosphate-buffered saline suspensions (7 log CFU/ml) to facilitate the attachment of 5.33 to 5.51 and 5.03 to 5.12 log CFU/cm(2), respectively, before they were immersed in TSB, IFB, or LJB, followed by incubation at 12 or 25 degrees C for up to 10 days. Biofilms were not produced at 12 degrees C. The number of cells of test strains increased by 1.42 to 1.67 log CFU/cm(2) and 1.16 to 1.31 log CFU/cm(2) in biofilms formed on stainless steel and feeding tubes, respectively, immersed in IFB at 25 degrees C; biofilms were not formed on TSB and LJB at 25 degrees C, indicating that nutrient availability plays a major role in processes leading to biofilm formation on the surfaces of these inert materials. These observations emphasize the importance of temperature control in reconstituted infant formula preparation and storage areas in preventing attachment and biofilm formation by E. sakazakii.