Organoid Models of Colorectal Pathology: Do They Hold the Key to Personalized Medicine? A Systematic Review.
Organoid Models of Colorectal Pathology: Do They Hold the Key to Personalized Medicine? A Systematic Review.
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DOI：
10.1097/dcr.0000000000001806
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发表时间：
2020-11
影响因子：
3.9
通讯作者：
Huang EH
中科院分区：
文献类型：
--
作者：
DeHaan RK;Sarvestani SK;Huang EH
补充数字内容可在文本中找到。结直肠癌和IBD占结直肠手术实践的很大一部分。历史研究模型为这些疾病的根本原因提供了见解，但存在许多局限性。本研究的目的是系统地回顾有关类器官模型在模拟良性和恶性结直肠病理学方面的优势的文献。来源包括PubMed、Ovid-Medline和奥维德Embase。两名评审员在2006年1月至2020年1月期间完成了对结肠和肠道类器官相关研究的文献系统综述。排除了未在人类或小鼠中进行的综述、评论、方案和研究。共纳入73篇文章。结直肠疾病的类器官模型越来越受欢迎，以进一步阐明这些疾病在个体水平的遗传、转录组和治疗反应。越来越复杂的模型，利用共培养技术正在迅速发展，允许在体外重演的疾病微环境。该综述仅为定性综述，由于类器官界缺乏充分利用的命名法，可能导致文章被排除。包括细胞系、患者源性肿瘤异种移植物和动物模型在内的历史疾病模型为我们理解结直肠病理学奠定了坚实的基础。以患者来源的上皮类器官和诱导的人肠类器官的形式的三维细胞培养的最新进展为在个体患者水平上进行病理学的高分辨率分析开辟了新的途径。最近的研究表明，类器官作为个性化医疗工具的潜力，它们能够保留患者的特征，包括治疗反应。
Supplemental Digital Content is available in the text. Colorectal cancer and IBD account for a large portion of the practice of colorectal surgery. Historical research models have provided insights into the underlying causes of these diseases but come with many limitations. The aim of this study was to systematically review the literature regarding the advantage of organoid models in modeling benign and malignant colorectal pathology. Sources included PubMed, Ovid-Medline, and Ovid Embase Two reviewers completed a systematic review of the literature between January 2006 and January of 2020 for studies related to colon and intestinal organoids. Reviews, commentaries, protocols, and studies not performed in humans or mice were excluded. A total of 73 articles were included. Organoid models of colorectal disease have been rising in popularity to further elucidate the genetic, transcriptomic, and treatment response of these diseases at the individual level. Increasingly complex models utilizing coculture techniques are being rapidly developed that allow in vitro recapitulation of the disease microenvironment. This review is only qualitative, and the lack of well utilized nomenclature in the organoid community may have resulted in the exclusion of articles. Historical disease models including cell lines, patient-derived tumor xenografts, and animal models have created a strong foundation for our understanding of colorectal pathology. Recent advances in 3-dimensional cell cultures, in the form of patient-derived epithelial organoids and induced human intestinal organoids have opened a new avenue for high-resolution analysis of pathology at the level of an individual patient. Recent research has shown the potential of organoids as a tool for personalized medicine with their ability to retain patient characteristics, including treatment response.