Relationship Between TH1/TH2 Cytokines and Immune Tolerance in Liver Transplantation in Rats
Relationship Between TH1/TH2 Cytokines and Immune Tolerance in Liver Transplantation in Rats
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DOI：
10.1016/j.transproceed.2008.08.014
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发表时间：
2008-10-01
影响因子：
0.9
通讯作者：
Gong, J.
中科院分区：
文献类型：
--
作者：
Chen, Y.;Chen, J.;Gong, J.
Objective.探讨Th 1/Th 2细胞因子表达与大鼠肝移植免疫耐受的关系。受体被分成三组（每组= 12只大鼠）。同种异体肝移植组采用雄性Wistar-to-Sprague-Dawley（SD）大鼠进行原位肝移植。SD-SD肝移植同系移植组与正常雄性SD大鼠假手术对照组相补充。我们评估了2周生存率、组织学变化以及Th 1/Th 2细胞因子：干扰素-γ（IFN-γ）和白细胞介素-10（IL-10）的血清和mRNA表达水平。同种异体移植组和同种异体移植组的1周存活率分别为100%和41.67%。2周生存率分别为75%和8.33%（P < .05）。术后1周，光镜检查发现同种异体移植物发生严重的急性排斥反应，而同系移植物组未见严重排斥反应。透射电镜下细胞形态学观察证实了这一点。血清IFN-γ通过酶联免疫吸附试验测定的同种异体移植物、同种异体移植物和对照组的Th 1细胞因子水平（pg/mL）分别为386.67 ± 14.36，159.83 ± 16.53和87.83 ± 8.67;对于IL-10（Th 2细胞因子），它们分别为126.33 +/- 13.10、288.33 +/- 17.10和70.50 +/- 7.23（P <0.05，同种异体移植物对同种异体移植物）。半定量逆转录聚合酶链反应（RT-PCR）结果显示，IFN-γ和IL-10 mRNA表达水平与血清水平相似。IFN-γ mRNA在同种异体移植组和IL-10 mRNA在同种异体移植组中表达更高（P <0.05）。Th 1/Th 2细胞因子的动态平衡在免疫耐受中起重要作用。Th 1向Th 2的偏移可能是免疫耐受的机制之一。
Objective. To explore the relationship between Th1/Th2 cytokine expressions and immune tolerance in rat liver transplantation.Methods. Recipients were divided into three groups (each group = 12 rats). The allograft group underwent orthotopic liver transplantation from male Wistar-to-Sprague-Dawley (SD) rats. The isograft group of SD-to-SD liver transplantion was complemented with a control group of normal male SD rats that had sham operations. We evaluated the 2-week survival rates histological changes, as well as serum and mRNA expression levels of Th1/Th2 cytokines: interferon-gamma (IFN-gamma) and interleukin-10 (IL-10).Results. The 1-week survival in the isograft and allograft groups were 100% and 41.67%, respectively. The 2-week survival rates were 75% and 8.33% (P < .05). Light microscopic inspection revealed severe acute rejection in the allograft but not the isograft group in the first week postoperation. This observation was verified by the cellular morphology tinder transmission electron microscopy. Serum IFN-gamma (Th1 cytokine) levels (pg/mL) determined by enzyme-linked immunosorbent assay in the allograft, isograft, and control groups were 386.67 +/- 14.36,159.83 +/- 16.53, and 87.83 +/- 8.67, respectively; for IL-10 (Th2 cytokine), they were 126.33 +/- 13.10, 288.33 +/- 17.10, and 70.50 +/- 7.23, respectively (P < .05, allograft vs isograft). The semiquantitative reverse-transcriptase polymerase chain reaction assay showed that expression of IFN-gamma and IL-10 mRNA was similar to that of serum levels. IFN-gamma mRNA was more highly expressed in the allograft group and IL-10 mRNA, in the isograft group (P < .05).Conclusions. The dynamic equilibrium of Th1/Th2 cytokines is critically involved in immune tolerance. The deviation of Th1 to Th2 may be one mechanism of immune tolerance.