68Ga-labelled DOTA-derivatised peptide ligands
68Ga-labelled DOTA-derivatised peptide ligands
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DOI：
10.1007/s00259-004-1486-0
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发表时间：
2004-08-01
影响因子：
9.1
通讯作者：
Hofmann, M
中科院分区：
文献类型：
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作者：
Meyer, GJ;Mäcke, H;Hofmann, M
Ge-68/Ga-68发生器提供了不依赖于回旋加速器的正电子发射断层扫描（PET）放射性药物。我们描述了一个系统，它允许安全和有效地处理Ge-68/Ga-68发生器洗脱液标记DOTA衍生的肽配体。该系统包括Ga-68标记物的浓缩和纯化以及肽的标记和纯化步骤，并且可以与不同的Ge-68/Ga-68发生器类型一起使用。用两种不同的DOTA衍生的生长抑素衍生物和DOTA衍生的蛙皮素衍生物测试了适用性和效率。10-20 nmol肽的量是足够的，并且导致所有肽的标记产率为50%。已证明内置的安全预防措施适用于允许将该方法用于常规临床应用。该系统由没有PET放射性药物制备经验的人员在“热实验室”中设置和操作。
Ge-68/Ga-68 generators provide cyclotron-independent access to positron emission tomography (PET) radiopharmaceuticals. We describe a system which allows the safe and efficient handling of Ge-68/Ga-68 generator eluates for labelling of DOTA-derivatised peptide ligands. The system comprises concentration and purification of the Ga-68 eluate as well as labelling and purification steps for peptides, and can be used with different Ge-68/Ga-68 generator types. The suitability and efficiency were tested with two different DOTA-derivatised somatostatin derivatives and a DOTA-derivatised bombesin derivative. Amounts of 10-20 nmol of the peptides were sufficient and resulted in labelling yields of 50% for all peptides. The built-in safety precautions have proven to be appropriate in allowing use of the method for routine clinical applications. The system was set up and operated in a "hot lab" by personnel with no previous experience in the preparation of PET radiopharmaceuticals.