A comparative study of the cytotoxicity of silver-based dressings in monolayer cell, tissue explant, and animal models
A comparative study of the cytotoxicity of silver-based dressings in monolayer cell, tissue explant, and animal models
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DOI：
10.1111/j.1524-475x.2006.00190.x
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Huang, Lin
中科院分区：
文献类型：
--
作者：
Burd, Andrew;Kwok, Chi H.;Huang, Lin
在过去的十年中，已经开发了各种先进的含银敷料。这些新制剂的结构、组成和银含量有相当大的变化。在本研究中，我们检查了五种市售的含银敷料（Acticoat（TM）、Aquacel（R）Ag、Contreet（R）Foam、PolyMem（R）银、Urgotul（R）SSD）。我们在单层细胞培养、组织外植体培养模型和小鼠切除伤口模型中评估了它们的细胞毒性。结果表明，Acticoat（TM）、Aquacel（R）Ag和Contreet（R）Foam在用特定溶质预处理时，可能对培养的角质形成细胞和成纤维细胞产生最显着的细胞毒性作用，而PolyMem（R）银和Urgotul（R）SSD表现出最小的细胞毒性。细胞毒性与通过培养基中银浓度测量的敷料释放的银相关。在评价表皮细胞增殖的组织外植体培养模型中，所有银敷料均导致上皮再生显著延迟。在小鼠切除伤口模型中，Acticoat（TM）和Contreet（R）Foam在创伤后第7天显示出对伤口上皮再生的强烈抑制。这些发现可能部分解释了使用某些局部银敷料后伤口愈合延迟或伤口上皮形成抑制的临床观察结果。在清洁的表面伤口（例如供体部位和表面烧伤）中使用含银敷料时以及将培养细胞应用于伤口时应谨慎。
Over the past decade, a variety of advanced silver-based dressings have been developed. There are considerable variations in the structure, composition, and silver content of these new preparations. In the present study, we examined five commercially available silver-based dressings (Acticoat (TM), Aquacel (R) Ag, Contreet (R) Foam, PolyMem (R) Silver, Urgotul (R) SSD). We assessed their cytotoxicity in a monolayer cell culture, a tissue explant culture model, and a mouse excisional wound model. The results showed that Acticoat (TM), Aquacel (R) Ag, and Contreet (R) Foam, when pretreated with specific solutes, were likely to produce the most significant cytotoxic effects on both cultured keratinocytes and fibroblasts, while PolyMem (R) Silver and Urgotul (R) SSD demonstrated the least cytotoxicity. The cytotoxicity correlated with the silver released from the dressings as measured by silver concentration in the culture medium. In the tissue explant culture model, in which the epidermal cell proliferation was evaluated, all silver dressings resulted in a significant delay of reepithelialization. In the mouse excisional wound model, Acticoat (TM) and Contreet (R) Foam indicated a strong inhibition of wound reepithelialization on the postwounding-day 7. These findings may, in part, explain the clinical observations of delayed wound healing or inhibition of wound epithelialization after the use of certain topical silver dressings. Caution should be exercised in using silver-based dressings in clean superficial wounds such as donor sites and superficial burns and also when cultured cells are being applied to wounds.