TRANSCRIPTIONAL ACTIVATION OF THE GENE FOR THE LARGE SUBUNIT OF HUMAN META-CALPAIN BY 12-ORTHO-TETRADECANOYL-PHORBOL-13-ACETATE
TRANSCRIPTIONAL ACTIVATION OF THE GENE FOR THE LARGE SUBUNIT OF HUMAN META-CALPAIN BY 12-ORTHO-TETRADECANOYL-PHORBOL-13-ACETATE
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DOI：
10.1016/0014-5793(92)80628-t
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发表时间：
1992-06-15
期刊：
影响因子：
3.5
通讯作者：
SUZUKI, K
中科院分区：
文献类型：
--
作者：
HATA, A;OHNO, S;SUZUKI, K
已经检查了用促进肿瘤的佛波酯 12-o-十四烷酰基佛波醇-13-乙酸酯 (TPA) 处理 HeLa 细胞对钙蛋白酶家族基因表达的影响。 在钙蛋白酶家族的 mRNA 中，只有人 m-钙蛋白酶大亚基 (钙蛋白酶 mL) 的 mRNA 通过 TPA 处理细胞而被特异性诱导，表明其在响应细胞刺激方面具有特定功能。 使用含有与细菌氯霉素乙酰转移酶（CAT）基因上游融合的钙蛋白酶mL基因启动子/增强子区域的融合基因进一步检查TPA对钙蛋白酶mL基因表达的影响，表明钙蛋白酶mL基因的启动子/增强子序列含有顺式作用元件，其响应TPA并激活下游序列的转录。
The effect of the treatment of HeLa cells with a tumor-promoting phorbol ester, 12-o-tetradecanoyl-phorbol-13-acetate (TPA) on the expression of the genes for the calpain family has been examined. Among the mRNAs for the calpain family, only the mRNA for the large subunit of human m-calpain (calpain mL) was specifically induced by treatment of cells with TPA, suggesting its specific function in response to cellular stimuli. The effect of TPA on the expression of the calpain mL gene was further examined using fusion genes containing the promoter/enhancer region of the calpain mL gene fused upstream of the bacterial chloramphenicol acetyltransferase (CAT) gene, showing that the promoter/enhancer sequence of the calpain mL gene contains a cis-acting element which responds to TPA and activates transcription of the downstream sequence.