Contractile proteins participate in release of erythroid growth regulators from mononuclear cells
Contractile proteins participate in release of erythroid growth regulators from mononuclear cells
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收缩蛋白参与单核细胞释放红细胞生长调节剂
DOI：
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
S. Kreczko
中科院分区：
文献类型：
--
作者：
N. Dainiak;M. Riordan;P. Strauss;L. Feldman;S. Kreczko
We have investigated the role of contractile proteins of circulating mononuclear cells in generation of membrane-associated, erythroid growth regulatory molecules. Lymphocytes and monocytes were incubated under serum-free conditions without and with cytochalasin B, cytochalasin D, or colchicine, and effects on positive and negative erythropoietic activities were determined in cell membranes and in surface membrane vesicle-rich pellets and supernatants of dialyzed medium conditioned by the cells. In serum-free cultures of human bone marrow, plasma membranes and exfoliated membrane-derived vesicles from cytochalasin-treated lymphocytes lost their capacity to support the formation of erythroid bursts, while monocyte membrane-associated inhibitory activity was abolished by preincubation with cytochalasin. In contrast, membrane-associated activities of colchicine-treated cells were unaffected. Cytochalasin-induced alterations of membrane regulatory molecules were observed in a dose-dependent fashion over a wide range of concentrations (1 to 100 micrograms/mL) tested. However, the capacity of membrane vesicle-free supernatants of medium conditioned by lymphocytes or monocytes was unaffected by cytochalasins, regardless of drug concentration used. Lysates of cytochalasin B-treated cells inhibited the activity of deoxyribonuclease I to a greater degree than did lysates of untreated cells, suggesting that the relative amount of monomeric actin is increased in the cytoplasm of treated cells. Furthermore, results of experiments with D-glucose and with cytochalasin D suggest that cytochalasin effects are independent of alterations in glucose metabolism. The data indicate that expression of plasma membrane- associated regulators is sensitive to agents that block polymerization of actin. They raise the possibility that changes in distribution of actin between unpolymerized and filamentous pools may influence the organization and/or function of mononuclear cell surface-associated erythroid regulatory molecules.
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DOI：
10.1073/pnas.84.19.6775
发表时间：
1987
影响因子：
11.1
作者：
Feldman,L;Cohen,CM;Riordan,MA;Dainiak,N
通讯作者：
Dainiak,N
单核细胞的表面膜囊泡刺激红系干细胞在培养物中增殖。
影响因子：
20.3
作者：
Dainiak,N;Cohen,CM
通讯作者：
Cohen,CM
从单核细胞中体外释放物理上可分离的因子，对红细胞生成产生相反的作用。
影响因子：
20.3
作者：
Feldman,L;Cohen,CM;Dainiak,N
通讯作者：
Dainiak,N
用抗膜抗体在体外中和红细胞爆发促进活性。
影响因子：
20.3
作者：
Dainiak,N;Feldman,L;Cohen,CM
通讯作者：
Cohen,CM