Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis
复制标题
DOI：
10.1128/jb.187.8.2810-2826.2005
复制
发表时间：
2005-04-01
影响因子：
3.2
通讯作者：
Hecker, M
中科院分区：
文献类型：
--
作者：
Höper, D;Völker, U;Hecker, M
枯草芽孢杆菌（Bacillus subtilis）依赖sigma(B)的一般应激调控有150多个成员。通过施加环境或代谢应激诱导这种调节，可使枯草芽孢杆菌的非生长、非孢子细胞具有多重、非特异性和先发制人的应激抗性。在这项研究中，我们进行了一项区域范围的表型筛选分析，以确定94个在一般应激调节候选成员中有缺陷的突变体的应激敏感性谱，这些突变体之前在我们对枯草芽孢杆菌一般应激反应的转录谱研究中被鉴定出来。表型筛选分析包括对生长抑制浓度乙醇（10%,vol/vol）或NaCl （10%, wt/vol）、严重热休克（54℃）和低温（4℃存活，12.5℃生长）的适应性分析。令人惊讶的是，85%的突变体测试显示灵敏度在et置信水平
The sigma(B)-dependent general stress regulon of Bacillus subtilis comprises more than 150 members. Induction of this regulon by imposition of environmental or metabolic stress confers multiple, nonspecific, and preemptive stress resistance to nongrowing, nonsporulated cells of B. subtilis. In this study we performed a region-wide phenotypic screening analysis to determine the stress sensitivity profiles of 94 mutants defective in candidate members of the general stress regulon that were previously identified in our transcriptional profiling study of the general stress response of B. subtilis. The phenotypic screening analysis included analysis of adaptation to a growth-inhibiting concentration of ethanol (10%, vol/vol) or NaCl (10%, wt/vol), severe heat shock (54 degrees C), and low temperature (survival at 4 degrees C and growth at 12.5 degrees C). Surprisingly, 85% of the mutants tested displayed increased sensitivity at an et confidence level of