Two classes of bipolar cell in the retina of the skate Raja erinacea
Two classes of bipolar cell in the retina of the skate Raja erinacea
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DOI：
10.1007/bf02284829
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发表时间：
1996-11-01
期刊：
影响因子：
--
通讯作者：
Chappell, RL
中科院分区：
文献类型：
--
作者：
Schlemermeyer, E;Chappell, RL
我们已经使用免疫反应对血清素和蛋白激酶C可视化两种不同类别的双极细胞在滑冰，拉贾erinacea的全杆视网膜。为了增强5 -羟色胺积累双极细胞的免疫反应，固定前，将一些视网膜在含有5 -羟色胺和pargyline的林格溶液中孵育。我们发现5 -羟色胺积累双极细胞的躯体位于内核层中线略远的地方。随着视条纹偏心率的增加，核周核的大小增加，同时核周核的分布密度下降。树突由粗壮的主茎发出，在到达外层丛状层之前向外分枝。轴突在内丛状层内分为两层，在层1和层2的边界和层4中有分支。聚积5 -羟色胺的双极细胞的整体形态与其他非哺乳动物的聚积5 -羟色胺的OFF双极细胞相似。蛋白激酶C免疫反应细胞表现为杆状双极细胞的典型外观。蛋白激酶C免疫反应性双极细胞的躯体呈梭形，位于血清素积累双极细胞的远端。这些双极性的树突在到达外层丛状层之前不分叉。蛋白激酶C免疫反应性双极细胞的细轴突位于网状层内第5层的大棒状末端，与哺乳动物的ON棒双极细胞的轴突末端有着惊人的相似性。我们的研究结果表明，滑冰的全杆视网膜包含至少两个不同的垂直通路，包括OFF双极细胞通路和经典的杆ON双极通路。
We have used immunoreactions against serotonin and protein kinase C to visualize two distinct classes of bipolar cell in the all-rod retina of the skate, Raja erinacea. To enhance the immunoreaction in serotonin-accumulating bipolar cells, prior to fixation, some retinas were incubated in Ringer's solution containing serotonin and pargyline. We found the somata of serotonin-accumulating bipolar cells to be located slightly distal to the midline of the inner nuclear layer. With increasing eccentricity from the visual streak, the size of the perikarya increases, concomitant with a decline in density of their distribution. Dendrites emanate from stout primary stalks and branch out before reaching the outer plexiform layer. Axons are bistratified within the inner plexiform layer with ramifications at the border of strata 1 and 2 and in stratum 4. The overall morphology of serotonin-accumulating bipolar cells is similar to that of serotonin-accumulating OFF bipolar cells of other nonmammalian vertebrates. Protein kinase C immunoreactive cells display the typical appearance of rod bipolar cells. Somata of protein kinase C immunoreactive bipolar cells are spindle-shaped and located distal to the serotonin-accumulating bipolar cells. Dendrites of these bipolars do not ramify before reaching the outer plexiform layer. Thin axons of protein kinase C immunoreactive bipolar cells end in large, club-shaped terminals in stratum 5 of the inner plexiform layer, bearing a striking similarity to axon terminals of mammalian ON rod bipolar cells. Our findings suggest that the all-rod retina of the skate contains at least two distinct vertical pathways including an OFF bipolar cell pathway in addition to a classical rod ON bipolar pathway.