Insights into the methanogenic degradation of N, N-dimethylformamide: The functional microorganisms and their ecological relationships
Insights into the methanogenic degradation of N, N-dimethylformamide: The functional microorganisms and their ecological relationships
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DOI：
10.1016/j.biortech.2018.09.074
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发表时间：
2019-01-01
影响因子：
11.4
通讯作者：
Li, Yu-You
中科院分区：
文献类型：
--
作者：
Kong, Zhe;Li, Lu;Li, Yu-You
采用厌氧消化污泥（ADS）、好氧活性污泥（AAS）和共培养污泥（CCS）对N， N-二甲基甲酰胺（DMF）的产甲烷降解进行了研究。阐明了DMF的代谢途径及在DMF产甲烷降解中起作用的微生物。由于缺乏DMF水解菌，ADS无法对DMF进行厌氧降解。AAS可以有效降解DMF，但在好氧条件下没有回收甲烷。在CCS中，DMF水解菌和产甲烷菌的共同培养使得DMF在厌氧条件下既能水解又能产甲烷，实现了DMF向甲烷的完全转化。然而，由于对中间体的竞争，生态位重叠降低了dmf水解细菌的丰度。硝酸盐的引入、及时补充AAS、微曝气和共消化可以维持dmf水解菌的高丰度，从而保证dmf的有效水解。
The methanogenic degradation of N, N-dimethylformamide (DMF) was investigated using anaerobic digested sludge (ADS), aerobic activated sludge (AAS) and co-cultured sludge (CCS), respectively. Both the metabolic pathway and the corresponding microorganisms which function in the methanogenic degradation of DMF were elucidated. DMF was unable to be degraded anaerobically by ADS due to the lack of DMF-hydrolyzing bacteria. DMF can be effectively degraded by AAS, however, no methane was recovered under the aerobic condition. The co-culture of DMF-hydrolyzing bacteria and methanogens in the CCS allowed for both hydrolysis of DMF and methane production to proceed successfully under the anaerobic condition, realizing the complete conversion from DMF to methane. However, a niche overlap due to the competition for the intermediates lowered the abundance of DMF-hydrolyzing bacteria. The introduction of nitrate, timely replenishment of AAS, micro-aeration and co-digestion were likely to maintain a high abundance of DMF-hydrolyzing bacteria to ensure an effective hydrolysis.