Cytokine Responses of Intestinal Epithelial-Like Caco-2 Cells to Non-Pathogenic and Opportunistic Pathogenic Yeasts in the Presence of Butyric Acid
Cytokine Responses of Intestinal Epithelial-Like Caco-2 Cells to Non-Pathogenic and Opportunistic Pathogenic Yeasts in the Presence of Butyric Acid
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DOI：
10.1271/bbb.70172
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发表时间：
2007-10
期刊：
影响因子：
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通讯作者：
S. Saegusa;M. Totsuka;S. Kaminogawa;T. Hosoi
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文献类型：
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作者：
S. Saegusa;M. Totsuka;S. Kaminogawa;T. Hosoi
白色念珠菌、酿酒酵母和它们的细胞壁组分酵母聚糖和葡聚糖已显示刺激用10 mM丁酸预培养的肠上皮细胞样Caco-2细胞产生白细胞介素-8（IL-8/CXCL-8）。在这项研究中，我们研究了这些酵母是否也改变了Caco-2细胞产生的其他细胞因子和环氧合酶（COX）。用10 mM丁酸和15% FBS培养Caco-2细胞4天，可提高编码IL-6、IL-8、IL-18、单核细胞趋化蛋白（MCP）-1、干细胞因子、转化生长因子（TGF）-β1、TGF-β3、肿瘤坏死因子（TNF）-α、考克斯-1和考克斯-2的mRNA的基础水平，而对粒细胞-巨噬细胞集落刺激因子（GM-CSF）和TGF-β2无影响。活S. cerevisiae或C.白念珠菌进一步增强IL-8的产生，但不增强其它细胞因子和COX的产生。非致病性酵母C. kefyr，C. utilis，C.乳酸克鲁维酵母（Kluyveromyces lactis）、乳酸克鲁维酵母（K. marxianus、粟酒裂殖酵母和鲁氏接合酵母，以及条件致病菌C. glabrata、光叶隐翅虫C. krusei，克鲁塞伊C. parapsilosis和C.从人组织样品中分离的热带假单胞菌也增强Caco-2细胞的IL-8分泌。
Candida albicans, Saccharomyces cerevisiae and their cell wall components, zymosan and glucan, have been shown to stimulate interleukin-8 (IL-8/CXCL-8) production by intestinal epithelial cell-like Caco-2 cells pre-cultured with 10 mM butyric acid. We examined in this study whether these yeasts also altered the production of other cytokines and cyclooxygenases (COXs) by Caco-2 cells. Culturing Caco-2 cells with 10 mM butyric acid and 15% FBS for 4 days enhanced the basal levels of mRNA encoding IL-6, IL-8, IL-18, monocyte chemoattractant protein (MCP)-1, stem cell factor, transforming growth factor (TGF)-β1, TGF-β3, tumor necrosis factor (TNF)-α, COX-1, and COX-2, but not of granulocyte-macrophage colony-stimulating factor (GM-CSF) and TGF-β2. The inclusion of live S. cerevisiae or C. albicans further enhanced the production of IL-8, but not of the other cytokines and COXs. The non-pathogenic yeasts, C. kefyr, C. utilis, C. versatilis, Kluyveromyces lactis, K. marxianus, Schizosaccharomyces pombe and Zygosaccharomyces rouxii, used for the production of fermented foods and probiotics, and the opportunistic pathogens, C. glabrata, C. krusei, C. parapsilosis and C. tropicalis, isolated from human tissue samples also enhanced IL-8 secretion by Caco-2 cells.