Global Proteomics Analysis of Protein Lysine Methylation.
Global Proteomics Analysis of Protein Lysine Methylation.
复制标题
蛋白质赖氨酸甲基化的全球蛋白质组学分析。
DOI：
10.1002/cpps.16
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发表时间：
2016-11-01
影响因子：
--
通讯作者：
Garcia BA
中科院分区：
文献类型：
--
作者：
Cao XJ;Garcia BA
Lysine methylation is a common protein post-translational modification dynamically mediated by protein lysine methyltransferases (PKMTs) and demethylases (PKDMs). Beyond histone proteins, lysine methylation on non-histone proteins play substantial roles in a variety of functions in cells, and is closely associated with diseases such as cancer. A large body of evidence indicates that the dysregulation of some PKMTs lead to tumorigenesis via their non-histone substrates. However, more studies on other PKMTs have made slow progress owing to the lack of the approaches for extensive screening of lysine methylation sites. Recently a series of publications to perform large-scale analysis of protein lysine methylation have emerged. In this unit, we introduce a protocol for the global analysis of protein lysine methylation in cells by means of immunoaffinity enrichment and mass spectrometry.
