Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes.
Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes.
复制标题
用于 ND-FISH 分析的寡核苷酸探针，用于鉴定黑麦和小麦染色体。
DOI：
10.1038/srep10552
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发表时间：
2015-05-21
影响因子：
4.6
通讯作者：
Tang Z
中科院分区：
文献类型：
--
作者：
Fu S;Chen L;Wang Y;Li M;Yang Z;Qiu L;Yan B;Ren Z;Tang Z
Genomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence in situ hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat × rye.
