Identification of hBD-3 in respiratory tract and serum: the increase in pneumonia
Identification of hBD-3 in respiratory tract and serum: the increase in pneumonia
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DOI：
10.1183/09031936.06.00105904
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发表时间：
2006-02-01
影响因子：
24.3
通讯作者：
Nakazato, M
中科院分区：
文献类型：
--
作者：
Ishimoto, H;Mukae, H;Nakazato, M
人β -防御素(hBD)-3是一种含有45个氨基酸的抗菌肽，最初从人皮肤中分离得到。RT-PCR也在气道中检测到hBD-3 mRNA。虽然hBD-3可能参与呼吸道内的抗菌防御，但hBD-3肽在呼吸系统中的存在尚未得到证实。采用径向扩散法和菌落计数法测定合成的hBD-3肽的抗菌活性。本文作者利用反相高效液相色谱（HPLC）和高灵敏度RIA，证实了人肺和血清匀浆中存在hBD-3肽。用免疫组织化学方法研究hBD-3肽的定位。采用RIA法测定血清hBD-3浓度。hBD-3表现出较强的抗菌活性，但不受盐浓度增加的影响。免疫组织化学方法观察了hBD-3在支气管和细支气管上皮细胞中的表达。细菌性肺炎患者血清hBD-3的平均+/- SD浓度在急性期为239.4 +/- 17.8 pg.mL(-1)，治疗结束后降至159.3+/-20.1 pg.mL（-1）。总之，这些发现将有助于阐明人β -防御素-3在宿主免疫应答中的作用，并确定该分子在呼吸道感染中的病理生理意义。
Human beta-defensin (hBD)-3, a 45 amino acid antimicrobial peptide, was originally isolated from human skin. hBD-3 mRNA has also been detected in the airways by RT-PCR. While hBD-3 may be involved in antimicrobial defences within the respiratory tract, the presence of hBD-3 peptide in the respiratory system has not yet been confirmed.The antimicrobial activity of the synthesised hBD-3 peptide was measured by a radial diffusion assay and a colony count assay. The present authors confirmed the presence of hBD-3 peptide in homogenates of human lung and serum using reverse-phase HPLC coupled with a highly sensitive RIA. The localisation of the hBD-3 peptide was investigated by immunohistochemistry. In addition, the serum concentrations of hBD-3 were measured by RIA.hBD-3 exhibited a strong antimicrobial activity, which was unaffected by increasing salt concentrations. Immunohistochemically, the current authors observed the expression of hBD-3 in bronchial and bronchiolar epithelial cells. The mean +/- SD serum concentration of hBD-3 in patients with bacterial pneumonia was 239.4 +/- 17.8 pg.mL(-1) in the acute phase and, decreased to 159.3+/-20.1 pg.mL(-1) after the completion of therapy.In conclusion, these findings will help elucidate the role of human beta-defensin-3 in host immune responses and identify the pathophysiological significance of this molecule in respiratory infections.