Oligoethylene glycols prevent thermal aggregation of α-chymotrypsin in a temperature-dependent manner : Implications for design guidelines.
Oligoethylene glycols prevent thermal aggregation of α-chymotrypsin in a temperature-dependent manner : Implications for design guidelines.
复制标题
低聚乙二醇以温度依赖性方式防止 α-胰凝乳蛋白酶的热聚集：对设计指南的影响。
DOI：
10.1002/btpr.1762
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发表时间：
2013
影响因子：
2.9
通讯作者：
Kentaro Shiraki
中科院分区：
文献类型：
--
作者：
Shunsuke Tomita;Yumiko Tanabe;Kentaro Shiraki
蛋白质聚集在许多领域都是有问题的，其中聚集在常规实验中经常发生。这项研究表明，四甘醇（TEG）和四甘醇二甲醚（TEGDE）作为聚集抑制剂，具有不同的独特性质，从典型的添加剂，以防止蛋白质聚集，如精氨酸（Arg）和NaCl。添加TEG和TEGDE均能很好地抑制α-糜蛋白酶的热聚集。有趣的是，当温度从65°C变化到85°C时，Arg和NaCl对热聚集的抑制作用几乎不变，而TEG和TEGDE都随着温度的升高而显著降低聚集速率。请注意，在75°C以上，TEG和TEGDE的效果高于Arg。TEG和TEGDE的这种温度依赖性行为为开发用于高温的聚集抑制剂提供了新的设计指导，即，环氧乙烷基团的重要性。© 2013美国化学工程师学会Biotechnol。程序：29：1325-1330，2013
Protein aggregation is problematic in various fields, where aggregation can frequently occur during routine experiments. This study showed that tetraethylene glycol (TEG) and tetraethylene glycol dimethyl ether (TEGDE) act as aggregation suppressors that have different unique properties from typical additives to prevent protein aggregation, such as arginine (Arg) and NaCl. Thermal aggregation of α‐chymotrypsin was well suppressed with the addition of both TEG and TEGDE. Interestingly, the suppressive effects of Arg and NaCl on thermal aggregation were almost unchanged when temperature was shifted from 65°C to 85°C, whereas both TEG and TEGDE significantly decreased the aggregation rate with increasing temperature. Note that the effects of TEG and TEGDE were higher than Arg above 75°C. This temperature‐dependent behavior of TEG and TEGDE provides a novel design guideline to develop aggregation suppressors for use at high temperature, i.e., the importance of the ethylene oxide group. © 2013 American Institute of Chemical EngineersBiotechnol. Prog., 29:1325–1330, 2013