Passive hemolysis by serum and cobra venom factor: a new mechanism inducing membrane damage by complement.
Passive hemolysis by serum and cobra venom factor: a new mechanism inducing membrane damage by complement.
复制标题
血清和眼镜蛇毒因子被动溶血：补体诱导膜损伤的新机制。
DOI：
10.1073/pnas.62.2.521
复制
发表时间：
1969
影响因子：
11.1
通讯作者：
H. Gewurz
中科院分区：
文献类型：
--
作者：
R. Pickering;M. Wolfson;R. Good;H. Gewurz
这里提出的研究表明，外源蛋白激活补体（C'）系统将导致膜损伤和反应时存在的未敏化红细胞的被动裂解。这些观察结果表明，除了经典的抗体-C'诱导的细胞溶解之外，还有其他途径或机制可以激活和参与末端C'成分产生细胞膜损伤。我们已经证明，源自眼镜蛇毒液并从聚丙烯酰胺上的单个蛋白带洗脱的物质可以在新鲜豚鼠血清存在的情况下促进未致敏的自体或异源红细胞的裂解，并且这种裂解诱导活性和 C' 抑制活性似乎存在于相同的级分中。裂解活性被已知损害经典C'3活性的几种试剂阻止，但不受干扰C'组分C'1和C'2功能的某些程序的影响，这表明该反应主要涉及C'3-C'9。此外，眼镜蛇毒 (CV) 因子会消耗眼镜蛇血清中的 C' 活性，并且 CV 因子（及其 5S 血清辅因子）将纯化的 C'3 转化为其无活性形式，(1) 表明该复合物与补体系统的反应在没有抗体参与的情况下发生。因此，由于 CV 因子的裂解诱导和 C' 抑制活性似乎是由与补体系统的类似相互作用引起的，因此这些观察结果表明，细胞膜损伤和细胞裂解可以通过补体系统的激活来完成，该机制涉及很少或不涉及经典抗体或 C' 组分 C'1、4 或 2。
The studies presented here indicate that activation of the complement (C') system by a foreign protein will cause membrane injury and passive lysis of unsensitized erythrocytes present at the time of the reaction. These observations suggest that in addition to the classical antibody-C'-induced cytolysis, there are alternative pathways or mechanisms for activation and participation of the terminal C' components in the production of cell membrane injury. We have shown that a substance derived from cobra venom and eluted from a single protein band on polyacrylamide can promote lysis of unsensitized autologous or heterologous erythrocytes in the presence of fresh guinea pig serum and that this lysis-inducing activity and C'-inhibiting activity appear to reside in the same fractions. The lytic activity is prevented by several agents known to impair classical C'3 activity, but is unaffected by certain procedures which interfere with the function of C' components C'1 and C'2, a suggestion that this reaction involves chiefly C'3-C'9. Further, the cobra venom (CV) factor depletes C' activity in cobra serum, and the CV factor (with its 5S serum cofactor) converts purified C'3 to its inactive form,(1) indicating that the reaction of this complex with the complement system occurs without participation of antibody. Therefore, since the lysis-inducing and C'-inhibiting activity of the CV factor appear to result from similar interactions with the complement system, these observations suggest that cell membrane damage and cell lysis can be accomplished through activation of the complement system by a mechanism involving little or no participation of classical antibody or C' components C'1, 4, or 2.