IMPACT OF PROLONGED FRACTION DELIVERY TIMES SIMULATING IMRT ON CULTURED NASOPHARYNGEAL CARCINOMA CELL KILLING
IMPACT OF PROLONGED FRACTION DELIVERY TIMES SIMULATING IMRT ON CULTURED NASOPHARYNGEAL CARCINOMA CELL KILLING
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模拟 IMRT 延长分次输送时间对培养鼻咽癌细胞杀伤的影响
DOI：
10.1016/j.ijrobp.2010.07.005
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发表时间：
2010-12-01
影响因子：
7
通讯作者：
Sun, Hen-Wen
中科院分区：
文献类型：
--
作者：
Zheng, Xiao-Kang;Chen, Long-Hua;Sun, Hen-Wen
目的研究模拟调强放疗（IMRT）的延长分段放射时间（FDT）对培养鼻咽癌（NPC）细胞杀伤的影响。本研究采用不同的方法和材料培养鼻咽癌细胞系CNE1和CNE2，用标准集落法评估模拟常规外束放疗和IMRT （FDT分别为15min、36min和50min）的分段放射方案的生物学效果。使用配对t检验检验不同方案辐照后细胞存活分数的差异。还通过剂量修正因子评估长时间fdt（从8到50分钟）对细胞杀伤的影响程度。估计的比较间歇性地交付2 Gy的有效性和持续交付1 5 - 2 GyResults CNE1和CNE2细胞存活分数后分次照射模拟强度都高于模拟常规体外放射治疗(p < 0 05)剂量的一小部分的剂量修改因素2 Gy从105增加到118从105年到111年对CNE1和CNE2的FDT被长时间从15到50吗本研究表明，模拟IMRT的延长FDT显著降低了CNE1和CNE2细胞系的细胞杀伤，并且随着FDT从15分钟延长到50分钟，这些负面影响会增加。如果这些影响得到体内和临床研究的证实，在设计鼻窦炎IMRT治疗时需要考虑(C) 2010 Elsevier Inc .
Purpose To determine the Impact of prolonged fraction delivery times (FDTs) simulating intensity modulated radiotherapy (IMRT) on cultured nasopharyngeal carcinoma (NPC) cell killingMethods and Material Cultured NPC cell lines CNE1 and CNE2 were used in this study The biological effectiveness of fractionated irradiation protocols simulating conventional external beam radiotherapy and IMRT (FDT of 15, 36, and 50 minutes) was estimated with standard colony assay, and the differences in cell surviving fractions after irradiation with different protocols were tested by use of the paired t test The Impact degree of prolonged FDTs (from 8 to 50 minutes) on cell killing was also assessed by the dose modifying factors, which were estimated by comparing the effectiveness of intermittently delivered 2 Gy with that of continuously delivered 1 5 to 2 GyResults The cell surviving fractions of both CNE1 and CNE2 after fractionated irradiation simulating IMRT were higher than those simulating conventional external beam radiotherapy (p < 0 05) The dose modifying factors for a fraction dose of 2 Gy increased from 105 to 118 for CNE1 and from 105 to 111 for CNE2 with the FDT being prolonged from 15 to 50 minutesConclusions This study showed that the prolonged FDTs simulating IMRT significantly decreased the cell killing in both CNE1 and CNE2 cell lines, and these negative effects increased with the FDT being prolonged from 15 to 50 minutes These effects, if confirmed by in vivo and clinical studies, need to be considered in designing IMRT treatments for NPC (C) 2010 Elsevier Inc