Dimensional and temporal controls of three-dimensional cell migration by zyxin and binding partners.
Dimensional and temporal controls of three-dimensional cell migration by zyxin and binding partners.
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DOI：
10.1038/ncomms1711
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发表时间：
2012-03-06
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
自发分子振荡在生物学中普遍存在。但据我们所知，尚未观察到周期性细胞迁移模式。在这里，我们报告的高度定期，周期性迁移的细胞沿着直线轨道内产生的三维矩阵，每个行程涵盖几个细胞的长度，表型，不发生在传统的基板。短发夹RNA耗竭表明，这些一维振荡是由zyxin和结合伴侣α-辅肌动蛋白和p130 Cas唯一控制的，而不是血管扩张剂刺激的磷蛋白和富含半胱氨酸的蛋白1。振荡概括为细胞迁移沿着一维微图案，但不是在二维顺应性基板。这些结果表明，虽然二维运动可以很好地描述的速度和持久性，三维运动需要两个额外的参数，矩阵中的细胞路径的维数和细胞运动的时间控制沿着这些路径。这些结果还表明，zyxin/α-actinin/p130 Cas模块可以确保三维基质中的运动细胞在最短的时间内探索最大的空间。
Spontaneous molecular oscillations are ubiquitous in biology. But to our knowledge, periodic cell migratory patterns have not been observed. Here we report the highly regular, periodic migration of cells along rectilinear tracks generated inside three-dimensional matrices, with each excursion encompassing several cell lengths, a phenotype that does not occur on conventional substrates. Short hairpin RNA depletion shows that these one-dimensional oscillations are uniquely controlled by zyxin and binding partners α-actinin and p130Cas, but not vasodilator-stimulated phosphoprotein and cysteine-rich protein 1. Oscillations are recapitulated for cells migrating along one-dimensional micropatterns, but not on two-dimensional compliant substrates. These results indicate that although two-dimensional motility can be well described by speed and persistence, three-dimensional motility requires two additional parameters, the dimensionality of the cell paths in the matrix and the temporal control of cell movements along these paths. These results also suggest that the zyxin/α-actinin/p130Cas module may ensure that motile cells in a three-dimensional matrix explore the largest space possible in minimum time.
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影响因子：
3.4
作者：
Kapustina, Maryna;Weinreb, Gabriel E.;Elston, Timothy C.
通讯作者：
Elston, Timothy C.
影响因子：
3.4
作者：
Costigliola, Nancy;Kapustina, Maryna T.;Jacobson, Ken
通讯作者：
Jacobson, Ken
影响因子：
4.8
作者：
Feng, Yunfeng;Zhao, Haibo;Longmore, Gregory D.
通讯作者：
Longmore, Gregory D.
DOI：
10.1083/jcb.200810041
发表时间：
2009-02-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Doyle AD;Wang FW;Matsumoto K;Yamada KM
通讯作者：
Yamada KM
粘着斑蛋白在三维细胞运动中的独特作用。
影响因子：
21.3
作者：
Fraley, Stephanie I.;Feng, Yunfeng;Krishnamurthy, Ranjini;Kim, Dong-Hwee;Celedon, Alfredo;Longmore, Gregory D.;Wirtz, Denis
通讯作者：
Wirtz, Denis