CAF01 liposomes as a mucosal vaccine adjuvant: In vitro and in vivo investigations
CAF01 liposomes as a mucosal vaccine adjuvant: In vitro and in vivo investigations
复制标题
DOI：
10.1016/j.ijpharm.2009.10.043
复制
发表时间：
2010-05-05
影响因子：
5.8
通讯作者：
Nielsen, Hanne Morck
中科院分区：
文献类型：
--
作者：
Christensen, Dennis;Foged, Camilla;Nielsen, Hanne Morck
与非肠道接种相比，粘膜接种疫苗有许多优势。与侵入性接种方法相比，无针黏膜接种具有操作简单、靶向多种病原体的入口点、降低感染其他病原体的风险等优点。CAF01是一种新型的脂质体疫苗佐剂，具有显著的免疫刺激活性。采用CALU-3上皮细胞体外培养模型，研究CAF01脂质体作为黏膜疫苗佐剂的可能性。因此，在CAF01暴露后，对抗原的粘膜通透性以及分化良好的细胞的上皮完整性和代谢活性进行了研究。结果表明，CAF01促进了抗原通过CALU-3细胞粘液层的传输，增加了细胞层中的抗原浓度，以及跨上皮细胞的传输。此外，CAF01对CALU-3细胞耐受性良好，体内研究表明，经鼻注射流感疫苗与CAF01联合应用后，小鼠的细胞免疫(CMI)和体液免疫反应都有所增加。因此，CAF01是一种很有前途的粘膜给药佐剂。(C)2009爱思唯尔B.V.保留所有权利。
Mucosal administration of vaccines has many advantages compared to parenteral vaccination. Needle-free mucosal vaccination would be easily applicable, target the vaccine to the entry point of many pathogens, and reduce the risk of infection with other pathogens during vaccination as compared to invasive methods.CAF01 is a novel liposome-based vaccine adjuvant with remarkable immunostimulatory activity. The potential of CAF01 liposomes as adjuvant for mucosal vaccines was investigated using the Calu-3 epithelial cell culture in vitro model. Thus, the mucosal permeability of the antigen as well as the epithelial integrity and the metabolic activity of the well-differentiated cells were investigated after exposure to CAF01. Finally, the adjuvant was tested for nasal administration in mice, combined with an influenza vaccine.The results suggest that CAF01 enhanced transport of antigen through the mucus layer on Calu-3 cells, increasing the concentration of antigen in the cell layer, as well as the transport across the epithelial cells. Furthermore CAF01 was well tolerated by the Calu-3 cells and the in vivo studies demonstrated increased cell-mediated immunity (CMI) as well as humoral immune responses in mice after nasal application of the influenza vaccine when combined with CAF01. CAF01 is thus a promising adjuvant for mucosal delivery. (C) 2009 Elsevier B.V. All rights reserved.