Rates of sterol synthesis and uptake in the major organs of the rat in vivo.
Rates of sterol synthesis and uptake in the major organs of the rat in vivo.
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发表时间：
1981-05
影响因子：
6.5
通讯作者：
S. Turley;J. Andersen;J. Dietschy
中科院分区：
文献类型：
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作者：
S. Turley;J. Andersen;J. Dietschy
本研究旨在测定雌性大鼠体内主要器官的甾醇合成和摄取速率。在光照周期的中暗阶段，对对照动物、通过输注乳糜微粒选择性抑制肝甾醇合成的动物和手术切除小肠和肝脏的动物给予[（3）H]水，1小时后测量不同器官中[（3）H]标记的毛地黄皂苷可沉淀甾醇（[（3）H]-DPS）的含量。在对照组动物中，[（3）H]DPS的最高含量见于肝脏（2279 nmol/hr/g）、肾上腺（1222）、卵巢（791）和小肠（529）：其他13个组织中新合成的甾醇含量要低得多。通过选择性抑制肝脏中的甾醇合成或通过手术切除小肠和肝脏，确定在整个动物中合成的[（3）H]DPS总量中，约50%发生在肝脏中，24%发生在小肠中，8%发生在皮肤中，18%发生在尸体的剩余组织中。通过分析血液中[（3）H]DPS含量与各器官中[（3）H] DPS含量之间的关系，可以进一步确定各组织中合成了多少[（3）H]DPS以及从血液中摄取了多少[（3）H]DPS。肾上腺摄取率最高，只有4%的[（3）H]DPS组织含量来自局部合成。在脾脏（6%）、肺（17%）和肾脏（26%）中也发现了相对于摄取速率的低合成速率。相比之下，在其他器官中，从血液中几乎没有摄取[（3）H]DPS，因此例如，脑和肌肉中存在的>75%的[（3）H]DPS是由于局部合成。降低血浆胆固醇的循环水平显著增加肾上腺、脾和肾等组织中[（3）H]DPS的合成，这些组织依赖血浆胆固醇作为组织固醇的主要来源，但在肌肉和脑等组织中不存在[（3）H] DPS。这些研究量化了每个主要器官对全身合成的重要性，并描绘了大鼠主要组织间[（3）H]DPS的运动速率。Turley，S. D、J. M. Andersen和J.M.迪奇大鼠体内主要器官中甾醇合成和摄取的速率。
This study was undertaken to determine the rates of sterol synthesis and uptake in the major organs of the female rat in vivo. At the mid-dark phase of the light cycle, control animals, animals in which hepatic sterol synthesis had been selectively inhibited by chylomicron infusion and animals in which the small intestine and liver had been surgically removed, were administered [(3)H]water, and the content of (3)H-labeled digitonin-precipitable sterols ([(3)H]-DPS) in different organs was measured 1 hr later. In control animals, the highest content of [(3)H]DPS was found in the liver (2279 nmol/hr per g), adrenal gland (1222), ovary (791), and small bowel (529): the content of newly synthesized sterols was much lower in 13 other tissues. By selectively inhibiting sterol synthesis in the liver or by surgically removing the small intestine and liver, it was determined that of the total amount of [(3)H]DPS synthesized in the whole animal about 50% had occurred in the liver, 24% in the small bowel, 8% in the skin, and 18% in the remaining tissues of the carcass combined. By analyzing the relationship between the content of [(3)H]DPS in blood and in each organ, it was further possible to determine how much [(3)H]DPS was synthesized and how much was taken up from the blood in each tissue. The highest rate of uptake was found in the adrenal gland where only 4% of the tissue content of [(3)H]DPS came from local synthesis. Low rates of synthesis relative to the rates of uptake, were also found in the spleen (6%), lung (17%), and kidney (26%). In contrast, in other organs there was little uptake of [(3)H]DPS from blood so that >75% of the [(3)H]DPS present in brain and muscle, for example, was due to local synthesis. Lowering the circulating levels of plasma cholesterol markedly increased the synthesis of [(3)H]DPS in tissues like adrenal gland, spleen, and kidney that were dependent upon plasma cholesterol as the major source of tissue sterols, but not in tissues such as muscle and brain. These studies have quantitated the importance of each major organ to total body synthesis and have delineated the rates of movement of [(3)H]DPS between major tissue compartments of the rat.-Turley, S. D., J. M. Andersen, and J. M. Dietschy. Rates of sterol synthesis and uptake in the major organs of the rat in vivo.