Flexible nanoporous tunable electrical double layer biosensors for sweat diagnostics.
Flexible nanoporous tunable electrical double layer biosensors for sweat diagnostics.
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DOI：
10.1038/srep14586
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发表时间：
2015-09-30
影响因子：
4.6
通讯作者：
Prasad S
中科院分区：
文献类型：
--
作者：
Munje RD;Muthukumar S;Panneer Selvam A;Prasad S
一种超灵敏和高度特异性的双电层（EDL）调制生物传感器，使用纳米多孔柔性基板可穿戴诊断证明与合成和人类汗液中的压力生物标志物皮质醇的检测。氧化锌薄膜用作与液体接触的活性区域，所述液体即含有生物分子的合成汗液和人汗液。汗液中的皮质醇检测通过测量和量化由于通过施加低正交方向的交流（AC）电场调节双电层内的双层电容而引起的阻抗变化来实现。在存在纳米多孔柔性基底的情况下放大在液体-半导体界面处形成的EDL，从而允许测量由于诊断相关浓度下的抗体-激素相互作用而引起的交流阻抗信号的变化。用这些新型生物传感器证明了合成汗液中1 pg/mL或2.75 pmol皮质醇和人体汗液中1 ng/mL皮质醇的高灵敏度检测。使用细胞因子IL-1β证明了合成汗液中的特异性。在10-200 ng/mL的浓度范围内证明了人汗液中的皮质醇检测。
An ultra-sensitive and highly specific electrical double layer (EDL) modulated biosensor, using nanoporous flexible substrates for wearable diagnostics is demonstrated with the detection of the stress biomarker cortisol in synthetic and human sweat. Zinc oxide thin film was used as active region in contact with the liquid i.e. synthetic and human sweat containing the biomolecules. Cortisol detection in sweat was accomplished by measuring and quantifying impedance changes due to modulation of the double layer capacitance within the electrical double layer through the application of a low orthogonally directed alternating current (AC) electric field. The EDL formed at the liquid-semiconductor interface was amplified in the presence of the nanoporous flexible substrate allowing for measuring the changes in the alternating current impedance signal due to the antibody-hormone interactions at diagnostically relevant concentrations. High sensitivity of detection of 1 pg/mL or 2.75 pmol cortisol in synthetic sweat and 1 ng/mL in human sweat is demonstrated with these novel biosensors. Specificity in synthetic sweat was demonstrated using a cytokine IL-1β. Cortisol detection in human sweat was demonstrated over a concentration range from 10–200 ng/mL.