Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography.
Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography.
复制标题
DOI：
10.14814/phy2.13437
复制
发表时间：
2017-09
影响因子：
2.5
通讯作者：
Hausenloy DJ
中科院分区：
文献类型：
--
作者：
Beikoghli Kalkhoran S;Hall AR;White IJ;Cooper J;Fan Q;Ong SB;Hernández-Reséndiz S;Cabrera-Fuentes H;Chinda K;Chakraborty B;Dorn GW 2nd;Yellon DM;Hausenloy DJ
先前已使用 2D 电子显微镜研究了线粒体融合蛋白 2 (MFN2) 缺乏对成人心脏中线粒体形态和线粒体-连接肌浆网 (jSR) 复合体的影响，但这种方法无法提供这些成像参数的 3D 空间评估。在这里，我们使用 3D 电子断层扫描显示 MFN2 缺陷的线粒体体积更大、更长、更圆。与 WT 线粒体相比，线粒体与 jSR 的接触较少，并且线粒体和 jSR 之间的距离增加。与 2D 电子显微镜相比，3D 电子断层扫描可以进一步了解成人心脏中的线粒体形态和线粒体-jSR 复合体。
The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria–junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2‐deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria–jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria–jSR complex in the adult heart.