Microcycle sporulation of Streptomyces viridochromogenes.
Microcycle sporulation of Streptomyces viridochromogenes.
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绿色产色链霉菌的微循环孢子形成。
DOI：
10.1007/bf00409764
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发表时间：
1984
影响因子：
2.8
通讯作者：
Ensign,JC
中科院分区：
文献类型：
--
作者：
Koepsel,R;Ensign,JC
产色链霉菌和产色链霉菌深层培养产孢子法。描述了天牛。激活后的孢子在复合培养基中萌发，然后转移到含有无机盐和2.5毫升精氨酸作为碳源和氮源的特定培养基上。产孢量在18-24小时之间同步发生。平均每个原始孢子可获得10个孢子。病毒致色基因。微循环形成的孢子在表面形态、休眠、萌发力、耐热性、耐溶菌酶和超声波等方面与表面培养的孢子几乎相同。微循环形成的孢子与表面孢子的海藻糖含量不同，海藻糖含量为1.3%，占干重的9.7%。
A procedure for submerged culture sporulation ofStreptomyces viridochromogenesandS. coelicoloris described. Acitvated spores were germinated in a complex medium and then shiftet to a defined medium containing mineral salts and 2.5 mML-arginine as carbon and nitrogen source. Sporulation occurred synchronously between 18–24h incubation. An average of 10 spores were obtained from each original spore inS. viridochromogenes. The microcycle formed spores were nearly identical to surface grown spores with respect to surface morphology, dormancy, germinability and resistance to heat, lysozyme and sonication. The microcycle formed spores differed from surface spores in containing significantly less trehalose, 1.3% compared to 9.7% of the dry weight.