Amino acid uptake is strongly affected during exponential growth of Saccharomyces cerevisiae in 0.7 M NaCl medium.
Amino acid uptake is strongly affected during exponential growth of Saccharomyces cerevisiae in 0.7 M NaCl medium.
复制标题
酿酒酵母在 0.7 M NaCl 培养基中指数生长期间，氨基酸摄取受到强烈影响。
DOI：
10.1111/j.1574-6968.1998.tb12810.x
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发表时间：
1998
影响因子：
2.1
通讯作者：
A. Blomberg
中科院分区：
文献类型：
--
作者：
J. Norbeck;A. Blomberg
用35 S-甲硫氨酸标记在0.7 M NaCl中生长的酿酒酵母，发现甲硫氨酸掺入蛋白质中降低了5-6倍，这不能仅仅归因于在0.7 M NaCl中生长的细胞的约50%更长的世代时间。随后的高亲和力蛋氨酸摄取系统的研究表明，在0.7 M NaCl培养基中生长期间，蛋氨酸的摄取强烈减少。这种减少的摄取被证明是应变无关的，主要是由约20倍降低的最大速度（Vmax）的运输系统，而底物亲和力（Km）显示只有轻微的变化。此外，证明了亮氨酸和组氨酸高亲和力摄取系统以及15种不同氨基酸的混合物的盐引起的摄取减少。因此，我们认为，减少氨基酸的摄取是一个普遍的现象，在盐生长的细胞。
Labelling of Saccharomyces cerevisiae grown in 0.7 M NaCl with 35S-methionine revealed a 5-6 fold lowering of the methionine incorporation into protein, which could not be attributed solely to the approximately 50% longer generation time of cells grown in 0.7 M NaCl. Subsequent studies of the high affinity methionine uptake system showed a strongly reduced uptake of methionine during growth in 0.7 M NaCl medium. This reduced uptake was shown to be strain-independent and caused mainly by an approximately 20-fold lowered maximum velocity (Vmax) of the transport system, while the substrate affinity (Km) displayed only a minor change. A salt-instigated reduction of uptake was furthermore demonstrated for the leucine and histidine high affinity uptake systems and also for a mixture of 15 different amino acids. We therefore suggest that the reduced amino acid uptake is a general phenomenon observed in salt-grown cells.