Inhibition of cartilage breakdown by hydrocortisone in a tissue culture model of rheumatoid arthritis.
Inhibition of cartilage breakdown by hydrocortisone in a tissue culture model of rheumatoid arthritis.
复制标题
在类风湿关节炎组织培养模型中氢化可的松抑制软骨破坏。
DOI：
10.1136/ard.42.3.323
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发表时间：
1983
影响因子：
27.4
通讯作者：
Sledge,CB
中科院分区：
文献类型：
--
作者：
Steinberg,JJ;Kincaid,SB;Sledge,CB
牛鼻软骨盘与人类风湿性关节炎滑膜或滑膜条件培养基共培养后，由于软骨分解而大量释放蛋白多糖。我们评估了氢化可的松对软骨和培养基之间蛋白多糖分布的影响，以及对软骨分解的影响，软骨分解表现为蛋白多糖或35 S-产物从预标记的椎间盘中释放。滑膜的存在抑制净蛋白多糖合成的能力，防止其在软骨中的积累，这是受氢化可的松的影响很小。药理浓度的氢化可的松的主要反应是抑制自发和滑膜诱导的软骨破裂。在培养过程中通常发生的滑膜蛋白的自溶也同样被相当剂量的皮质类固醇所阻止。从软骨中释放的35 S-标记的降解产物的色谱分布的变化符合皮质类固醇诱导的内源性溶酶体或相关蛋白酶活性的抑制。此外，滑膜中负责软骨细胞介导的软骨破坏的早期事件的抑制可能对皮质类固醇的总体作用有显著贡献。
Bovine nasal cartilage discs cocultured with human rheumatoid synovial membrane or synovial-membrane-conditioned media release proteoglycan largely as a result of cartilage breakdown. We assessed the effects of hydrocortisone on proteoglycan distribution between cartilage and culture medium, and on cartilage breakdown expressed as the release of either proteoglycan or 35S-products from prelabelled discs. The presence of synovial membrane inhibited the capacity for net proteoglycan synthesis, preventing its accumulation in cartilage; this was little affected by hydrocortisone. The major response to pharmacological concentrations of hydrocortisone was suppression of both spontaneous and synovial-membrane-induced cartilage breakdown. The autolysis of synovial protein that normally occurred during culture was similarly prevented by comparable doses of corticosteroid. Changes in chromatographic distribution of the 35S-labelled degradation products released from cartilage conformed with a corticosteroid-induced inhibition of endogenous lysosomal or related proteinase activity. Additionally, inhibition of the early events in synovial membrane that are responsible for chondrocyte-mediated breakdown of cartilage may contribute significantly to the overall corticosteroid effect.