Diaporthe species associated with peach tree dieback in Hubei, China
Diaporthe species associated with peach tree dieback in Hubei, China
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DOI：
10.5943/mycosphere/8/5/2
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发表时间：
2017-01-01
期刊：
影响因子：
14.6
通讯作者：
Yan, J. Y.
中科院分区：
文献类型：
--
作者：
Dissanayake, A. J.;Zhang, W.;Yan, J. Y.
桃树病有多种症状和病因。在我国桃园中，仅报道过与桃树伴生的Botryosphaeriaceae分类群。摘要本研究旨在对湖北省农业科学院金水实验果园桃树伴生的桃蚜进行鉴定和鉴定。这些真菌是从有渗出物的病桃干和芽中分离出来的。利用形态学和病原学特征，结合内部转录间隔物（ITS）、部分翻译延伸因子1- α (ef1 - α)、β -微管蛋白（BT）和钙调蛋白（CAL）序列进行系统发育分析，完成真菌鉴定。62份病样共分离得到48株散孔虫，其中以散孔虫居多（69%），其次为momicola sp.（12.5%）、pescicola sp.（10%）和taoicola sp.（8.5%）。所有鉴定的品种在接种到离体桃枝上时都能引起不同程度的坏死损伤
Peach tree diseases have a variety of symptoms and causes. Only Botryosphaeriaceae taxa have been reported in association with peach trees in Chinese peach orchards. This study aims to identify and characterize Diaporthe species associated with peach trees in Jinshui Experimental Orchard in Hubei Academy of Agriculture Sciences, Hubei Province, China. The fungi were isolated from diseased peach trunks and shoots showing exudates. Fungal identification was accomplished using a combination of morphological and pathogenic characteristics together with phylogenetic analyses based on internal transcribed spacer (ITS), partial translation elongation factor 1-alpha (EF1-alpha), beta-tubulin (BT) and calmodulin (CAL) sequences. A total of 48 Diaporthe isolates were obtained from 62 diseased samples and most isolates were identified as Diaporthe eres (69 %), followed by D. momicola sp. nov (12.5 %), D. pescicola sp. nov. (10 %) and D. taoicola sp. nov. (8.5 %). All identified species were able to cause necrotic lesions at different levels of severity when inoculated into detached peach shoots