Postnatal development of some membrane-bound enzymes of rat liver and kidney.
Postnatal development of some membrane-bound enzymes of rat liver and kidney.
复制标题
大鼠肝脏和肾脏的一些膜结合酶的出生后发育。
DOI：
10.1016/0304-4165(81)90278-6
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
P. Goldsmith
中科院分区：
文献类型：
--
作者：
P. Goldsmith
1.大鼠肝脏酰基辅酶A的开发：锡-甘油-3-磷酸-O-酰基转移酶（EC 2.3. 1.15）的特点是在新生儿期活动的增加和减少，其次是第二次增加和减少在后期断奶期。肾脏酰基转移酶在新生儿期表现出类似的活性峰值，然后在断奶后期增加至成人活性水平。2.核苷二磷酸酶活性在新生儿期迅速增加，此后在肝脏和肾脏中逐渐升高至成人水平。酶的潜伏期在出生后迅速增加，此后随年龄变化不大。在研究的所有年龄组中，该酶在肝脏中的潜伏性似乎比在肾脏中的潜伏性更强。3.肝脏NADPH-细胞色素c还原酶在新生儿期有一个陡峭的最大值和最小值，然后在断奶后期再次增加到成人水平。肾脏中的酶显示出类似的发育模式，但比活性水平低得多。4. SN-甘油-3-磷酸酰基转移酶活性显着高于在整个新生期的快速平滑膜增殖的粗糙膜比平滑膜。这种酶活性的分布与其他人在苯巴比妥诱导的光滑膜增殖中报道的不同，并表明新生儿期磷脂合成中粗糙膜的主要作用。5.这些酶中的每一种在粗糙和光滑微粒体亚组分的发展中的定性相似性与先前报道的葡萄糖-6-磷酸酶的结果形成鲜明对比。
1. The development of rat liver acyl-CoA: sn-glycerol-3-phosphate-O-acyl-transferase (EC 2.3. 1.15) is characterized by an increase and decrease in activity during the neonatal period, followed by a second increase and decrease during the late weaning period. Kidney acyltransferase exhibits a similar peak in activity during the neonatal period before increasing to adult levels of activity during the late weaning period. 2. Nucleosidediphosphatase activity increases rapidly during the neonatal period and thereafter gradually rises to adult levels in both liver and kidney. The latency of the enzyme increases rapidly after birth and thereafter shows little change with age. The enzyme appears to be more latent in the liver than in the kidney at all ages studied. 3. NADPH-cytochrome c reductase of liver has a single steep maximum and minimum in activity during the neonatal period, before increasing again to adult levels during the late weaning period. The enzyme in kidney shows a similar developmental pattern but at much lower levels of specific activity. 4. sn-Glycerol-3-phosphate acyltransferase activity was significantly higher in rough than in smooth membranes throughout the neonatal period of rapid smooth membrane proliferation. This distribution of enzyme activity is unlike that reported by others in phenobarbital-induced smooth membrane proliferation and suggests a major role for rough membranes in phospholipid synthesis during the neonatal period. 5. The qualitative similarity in development in rough and smooth microsomal subfractions for each of these enzymes is in distinct contrast with results previously reported for glucose-6-phosphatase.