Escherichia coli one- and two-hybrid systems for the analysis and identification of protein-protein interactions
Escherichia coli one- and two-hybrid systems for the analysis and identification of protein-protein interactions
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DOI：
10.1006/meth.1999.0908
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发表时间：
2000-01-01
期刊：
影响因子：
4.8
通讯作者：
Hochschild, A
中科院分区：
文献类型：
--
作者：
Hu, JC;Kornacker, MG;Hochschild, A
基于融合蛋白的遗传方法允许将遗传方法的力量应用于蛋白质或蛋白质片段的自组装，而不管融合组装结构域的正常功能是否已知或是否适合选择或筛选。最初由S. Fields和O. Song （1989, Nature 34,245 -246）描述的酵母双杂交系统变体的广泛采用证明了这些检测方法的有效性。这篇综述描述了许多用于选择或筛选基于在细菌中表达的杂交蛋白对报告蛋白构建的调控的蛋白-蛋白相互作用的系统，包括最近实现的用于大肠杆菌的可推广的双杂交系统。(C) 2000年学术出版社。
Genetic methods based on fusion proteins allow the power of a genetic approach to be applied to the self-assembly of proteins or protein fragments, regardless of whether or not the normal function of the fused assembly domains is either known or amenable to selection or screening. The widespread adoption of variations of the yeast two-hybrid system originally described by S. Fields and O. Song (1989, Nature 340, 245-246) demonstrates the usefulness of these kinds of assays. This review describes some of the many systems used to select or screen for protein-protein interactions based on the regulation of reporter constructs by hybrid proteins expressed in bacteria, including recent implementations of generalizable two-hybrid systems for Escherichia coli. (C) 2000 Academic Press.