Identification of AaCASPS7, an effector caspase in Aedes albopictus
Identification of AaCASPS7, an effector caspase in Aedes albopictus
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白纹伊蚊中效应子半胱天冬酶 AaCASPS7 的鉴定
DOI：
10.1016/j.gene.2016.08.008
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发表时间：
2016-11-15
期刊：
影响因子：
3.5
通讯作者：
Liu, Qingzhen
中科院分区：
文献类型：
--
作者：
Feng, Lingyan;Liu, Hao;Liu, Qingzhen
白纹伊蚊是一种传播各种虫媒病毒的媒介，由于其在全球范围内的迅速传播，正在成为对公众健康的重大威胁。一些报告表明，细胞凋亡可能是蚊子感染虫媒病毒的限制因素。因此，明确蚊虫体内的细胞凋亡途径，研究细胞凋亡与病毒感染的关系具有重要意义。细胞凋亡是一种程序性细胞死亡，在免疫、发育和组织动态平衡中起着至关重要的作用。半胱氨酸天冬氨酸氨基转移酶是一类保守的蛋白水解酶家族，在细胞凋亡中发挥重要作用。在本研究中，我们鉴定了白纹伊蚊AaCASPS7，这是一个与DIP-Teran昆虫Drice同源基因有很高同源性的caspase。系统发育分析表明，AaCASPS7与埃及伊蚊AeCASPS7有较近的亲缘关系。AaCASPS7显示了效应器caspase的几个典型特征，并显示了对效应器caspase底物的显著活性。Aacasps7转录本在白纹伊蚊发育和成虫阶段普遍表达。瞬时表达AaCASPS7诱导C6/36细胞caspase依赖性凋亡。综合以上数据，本研究确定了一个新的caspase，AaCASPS7，它可能是一个具有凋亡功能的caspase。进一步研究AaCASPS7的功能有助于更好地了解白纹伊蚊的细胞凋亡机制。(C)2016爱思唯尔B.V.保留所有权利。
Aedes albopictus mosquito is a vector of various arboviruses and is becoming a significant threat to public health due to its rapid global expansion. Several reports suggest that apoptosis could be a factor limiting arbovirus infection in mosquitoes. Thus, it is significant to identify apoptosis pathway and study the correlation between apoptosis and virus infection in mosquitoes. Apoptosis is a type of programmed cell death that plays a vital role in immunity, development, and tissue homeostasis. Caspases are a family of conserved proteases playing important roles in apoptosis. In this study, we identified Aedes albopictus AaCASPS7, a caspase shared high identity with dip-teran insect drICE orthologs. Phylogenetic analysis showed the closest relative of AaCASPS7 was Aedes aegypti AeCASPS7. AaCASPS7 displayed several features that were typical of an effector caspase and showed significant activity to effector caspase substrates. Aacasps7 transcripts were expressed ubiquitously in developmental and adult stages in Aedes albopictus mosquitoes. Transient expression of AaCASPS7 induced caspase-dependent apoptosis in C6/36 cells. Taken together the above data, this study identified a novel caspase, AaCASPS7, which might function as an apoptotic caspase. Further study the function of AaCASPS7 would facilitate better understanding the apoptotic mechanism in Aedes albopictus mosquito. (C) 2016 Elsevier B.V. All rights reserved.