Unreduced diploid nuclei in Cupressus dupreziana A. Camus pollen
Unreduced diploid nuclei in Cupressus dupreziana A. Camus pollen
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DOI：
10.1007/s001220051518
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发表时间：
2000-09
影响因子：
5.4
通讯作者：
C. Pichot;M. El Maâtaoui
中科院分区：
文献类型：
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作者：
C. Pichot;M. El Maâtaoui
杜普雷齐亚纳花粉的大小和 DNA 含量。 Camus是一种高度濒危的地中海针叶树，通过细胞形态学观察和流式细胞术分析对其进行评估，然后与C进行比较。 sempervirensL.花粉。成熟C. duprezianapollen 由两种大小不同的无核花粉粒组成。大约 35% 的颗粒表现出与 C 相似的尺寸。 sempervirenspollen，而 65% 的直径较大。然而，流式细胞术仅检测到一个荧光峰。 DNA含量C. dupreziana花粉的DNA含量是C. dupreziana花粉的两倍。永生花粉。成熟花粉和萌发花粉DNA含量的比较表明，两个物种的成熟花粉均停滞在G2期。与体细胞组织 (2C) 的 DNA 含量进行比较，为 C 中产生未减少的花粉提供了证据。 dupreziana。裸子植物的这一意想不到的特征是根据该物种的繁殖策略进行讨论的。
Size and DNA content of pollen ofCupressus duprezianaA. Camus,a highly endangered Mediterranean conifer, were assessed by cytomorphological observations and flow cytometric analyses and then compared toC. sempervirensL. pollen. MatureC. duprezianapollen was composed of two uninucleated types of pollen grains differing in size. Around 35% of the grains exhibited a size similar toC. sempervirenspollen, while 65% exhibited a larger diameter. However only one peak of fluorescence was detected by flow cytometry. DNA content ofC. duprezianapollen was twice the DNA content ofC. sempervirenspollen. Comparison of DNA contents of mature and germinating pollen revealed that mature pollen of both species were arrested in the G2 phase. Comparison with the DNA content of somatic tissue (2C) provided evidence for the production of unreduced pollen inC. dupreziana.This unexpected feature in gymnosperms is discussed in terms of reproductive strategy of this species.