Investigation on the relationship between cannabinoid CB1 and opioid receptors in gastrointestinal motility in mice
Investigation on the relationship between cannabinoid CB1 and opioid receptors in gastrointestinal motility in mice
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DOI：
10.1038/sj.bjp.0706824
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发表时间：
2006-08-01
影响因子：
7.3
通讯作者：
Hungund, Basalingappa L.
中科院分区：
文献类型：
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作者：
Carai, Mauro A. M.;Colombo, Giancarlo;Hungund, Basalingappa L.
1本研究调查了（a）大麻素CB 1受体敲除（CB 1-/-）小鼠是否表现出胃肠道转运改变和（B）大麻素CB 1和阿片受体在胃肠道转运调节中是否功能性相互作用。2通过全胃肠道转运评估胃肠道转运，测量胃内给药标记物的排泄时间3 CB 1-/-和纯合子CB 1-/-（CB 1-/-）小鼠在全肠和小肠转运中没有差异。CB 1-/-和CB 1-/-小鼠对吗啡的抑制作用同样敏感（10 mg kg（-1））和洛哌丁胺（3 mg kg（-1））。4此外，在CD 1小鼠中，大麻素CB 1受体拮抗剂利莫那班（0-0.5 mg kg-1）不能阻断吗啡（0-1.25 mg kg-1）和洛哌丁胺（0-0.5 mg kg-1）对小肠和全肠转运的抑制作用。同样，阿片受体拮抗剂纳洛酮（0-1 mg kg（-1））和纳洛酮（0 - 10 mg kg（-1）），未能阻断大麻素WIN 55的抑制作用，212-2（0-3 mg kg（-1））在小的和完整的大鼠中转运。5这些结果表明：（a）CB 1-/-小鼠克服内源性大麻素系统抑制功能的缺乏;（B）大麻素和阿片样物质受体系统在调节小鼠胃肠转运中没有相互作用。
1 This study investigated whether (a) cannabinoid CB1 receptor knockout (CB1-/-) mice displayed altered gastrointestinal transit and (b) cannabinoid CB1 and opioid receptors functionally interact in the regulation of gastrointestinal transit.2 Gastrointestinal transit was assessed by the Whole Gastrointestinal Transit, measuring the excretion time of an intragastrically administered marker ( whole intestine), and the Upper Gastrointestinal Transit, measuring the distance covered by the marker in the small intestine.3 CB1-/- and homozygous CB1-/- (CB1-/-) mice did not differ in both whole gut and small intestine transit. CB1-/- and CB1-/- mice were equally responsive to the inhibitory effect of morphine (10 mg kg(-1)) and loperamide (3 mg kg(-1)) on whole gut transit.4 Additionally, in CD1 mice the cannabinoid CB1 receptor antagonist, rimonabant (0-0.5 mg kg(-1)), failed to block the inhibitory effect of morphine (0-1.25 mg kg(-1)) and loperamide (0-0.5 mg kg(-1)) on transit in small and whole intestine. Similarly, the opioid receptor antagonists, naloxone (0-1 mg kg(-1)) and naltrexone ( 0 - 10 mg kg(-1)), failed to block the inhibitory effect of the cannabinoid WIN 55,212-2 (0-3 mg kg(-1)) on transit in small and whole intestine.5 These results suggest that (a) compensatory mechanisms likely developed in CB1-/- mice to overcome the lack of inhibitory function of endocannabinoid system; (b) cannabinoid and opioid receptor systems did not interact in regulating gastrointestinal transit in mice.