Cohesins bind to preferential sites along yeast chromosome III, with differential regulation along arms versus the centric region
Cohesins bind to preferential sites along yeast chromosome III, with differential regulation along arms versus the centric region
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DOI：
10.1016/s0092-8674(00)81019-3
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发表时间：
1999-07-23
期刊：
影响因子：
64.5
通讯作者：
Kleckner, N
中科院分区：
文献类型：
--
作者：
Blat, Y;Kleckner, N
姐妹染色单体内聚是由进化保守的染色体蛋白介导的，称为“内聚蛋白”。利用扩展的染色质免疫沉淀，我们分析了黏结蛋白Mcd1/Scc1和Smc1沿酵母染色体III的分布。这两种蛋白优先发生在相同的相似的23个位置。着丝粒周围大约50kb的区域会发出特别强烈的信号。突出的中心区结合似乎是从一个更均匀的分布中出现的，可能是由于染色体臂上黏结蛋白的差异损失。黏结蛋白结合峰与高局部AT组成峰紧密对应，碱基组成周期约为15 kb，与碱基组成等位周期约为50 kb不同，与黏结蛋白的轴向关联一致。所描述的方法可用于分析任何dna结合蛋白的分布，并通过微芯片沿着整个基因组进行分析。
Sister chromatid cohesion is mediated by evolutionary conserved chromosomal proteins, termed "cohesins." Using an extension of chromatin immunoprecipitation, we have analyzed the distribution of cohesins Mcd1/Scc1 and Smc1 along yeast chromosome III. Both proteins occur preferentially at the same similar to 23 positions. Sites in a similar to 50 kb region around the centromere give especially intense signals. Prominent centric region binding appears to emerge from a more even distribution, probably by differential loss of cohesins along the chromosome arms. Cohesin binding peaks correspond closely to peaks of high local AT composition, a base composition periodicity of similar to 15 kb that is distinct from the similar to 50 kb periodicity of base composition isochores, consistent with axis association of cohesins. The methodology described can be used to analyze the distribution of any DNA-binding protein and, via microchips, along entire genomes.