Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms
Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms
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DOI：
10.1128/aem.02073-07
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发表时间：
2008-01-01
影响因子：
4.4
通讯作者：
Kaplan, Jeffrey B.
中科院分区：
文献类型：
--
作者：
Izano, Era A.;Amarante, Matthew A.;Kaplan, Jeffrey B.
由于耐药菌株的传播，金黄色葡萄球菌和表皮葡萄球菌是人类日益重要的主要病原体。有证据表明，形成基质包裹的生物膜的能力有助于金黄色葡萄球菌和表皮葡萄球菌的发病。在这项研究中，我们研究了两种葡萄球菌生物膜基质聚合物：聚N-乙酰氨基葡萄糖表面多糖(PNAG)和胞外DNA(EcDNA)的功能。我们在96孔板上测定了PNAG降解酶(分散素B)和DNase I抑制生物膜形成、分离预先形成的生物膜以及使生物膜对阳离子洗涤剂十六烷基吡啶(CPC)的杀灭敏感的能力。当添加到生长介质中时，分散剂B和DNase I都能抑制金黄色葡萄球菌和表皮葡萄球菌生物膜的形成。DNase I能分离金黄色葡萄球菌生物膜，但不能分离金黄色葡萄球菌生物膜。同样，分散剂B使表皮葡萄球菌生物膜对CPC杀伤敏感，而DNase I使金黄色葡萄球菌生物膜对CPC杀伤敏化。我们得出结论，PNAG和ecDNA在金黄色葡萄球菌和表皮葡萄球菌生物膜中发挥着根本不同的结构作用。
Staphylococcus aureus and Staphylococcus epidermidis are major human pathogens of increasing importance due to the dissemination of antibiotic-resistant strains. Evidence suggests that the ability to form matrix-encased biofilms contributes to the pathogenesis of S. aureus and S. epidermidis. In this study, we investigated the functions of two staphylococcal biofilm matrix polymers: poly-N-acetylglucosamine surface polysaccharide (PNAG) and extracellular DNA (ecDNA). We measured the ability of a PNAG-degrading enzyme (dispersin B) and DNase I to inhibit biofilm formation, detach preformed biofilms, and sensitize biofilms to killing by the cationic detergent cetylpyridinium chloride (CPC) in a 96-well microtiter plate assay. When added to growth medium, both dispersin B and DNase I inhibited biofilm formation by both S. aureus and S. epidermidis. Dispersin B detached preformed S. epidermidis biofilms but not S. aureus biofilms, whereas DNase I detached S. aureus biofilms but not S. epidermidis biofilms. Similarly, dispersin B sensitized S. epidermidis biofilms to CPC killing, whereas DNase I sensitized S. aureus biofilms to CPC killing. We concluded that PNAG and ecDNA play fundamentally different structural roles in S. aureus and S. epidermidis biofilms.